天然产物研究与开发2010，Vol.22Issue(5)：769-776,8.
桂皮醛对小鼠柯萨奇病毒诱发病毒性心肌炎的作用
Influence of Cinnamaldehyde on Coxsackievirus B3-Induced Viral Myocarditis in Mice
摘要
Abstract
It has been demonstrated that Toll-like receptors 4 (TLR4) and their downstream signaling components play a critical role in coxsackievirns-mediated myocarditis, but their therapeutic acation remains indefinite. Cinnamaldehyde is known to possess anti-viral action and inhibite dose dependent NF-κB activation induced by TLR4 or wild-type TLR4.However,little is known regarding the effects of cinnamaldehyde on coxsackievirus B3 (CVB3)-induced viral myocardi-tis. Our studies showed that IC50 value of cinnamaldehyde in normal myocardial cells was only 15 μM in vitro. And cin-namaldehyde can significantly inhibit virus titre (P < 0.01) in myocardial cells by the concentration of 100-1000 μM for 72 hours. And cell survival rate has no difference in statistics between CVB group and cirmamaldehyde group (p 0.01). However,compared with model group,in viral myocarditis mice, cinnamaldehyde(20 and 40 mg/kg i. p. for 7 days) significantly reduced the content of NO in blood serum and such proteins' expression as inducible nitric oxide syn-thase (iNOS) ,tumor necrosis factor(TNF-a), nuclear factor-κB P65 (NF-κB P65)and TLR4(P < 0.05). It also low-ered the ratio of Hw/Bw after 21days and improved survival rate of mice and relieved pathological injury. These results indicated that though cinnamaldehyde had no antiviral activity in vitro, it can lower virus titer and depress TLR-4-NF-κB signal transmission. So we can say that cinnamaldehyde has a therapeutic effect on viral myocarditis mice. Through inhib-iting TLR-4-NF-κB signal transmission cinnamaldelyde may be a new method for treating viral myocarditis.关键词
桂皮醛/抗病毒/柯萨奇病毒B3/病毒性心肌炎/Toll样受体4Key words
Cinnamaldehyde/Anti-viral/CVB3/Viral Myocarditis/TLR4分类
医药卫生引用本文复制引用
丁媛媛,赵钢涛,杨凡,王四旺..桂皮醛对小鼠柯萨奇病毒诱发病毒性心肌炎的作用[J].天然产物研究与开发,2010,22(5):769-776,8.基金项目
This work was supported by the grant from the Nation-al Natural Science Foundation of China (20872180) and Traditional Chinese Medicine Administration of Shaanxi Province. （20872180）
