首页|期刊导航|中国神经再生研究（英文版）|Xiaoyaosan-containing serum inhibits corticosterone-induced apoptosis in PC12 cells by downregulating[Ca2+]i overload
中国神经再生研究（英文版）2010，Vol.5Issue(18)：1396-1402,7.DOI:10.3969/j.issn.1673-5374.2010.18.006
Xiaoyaosan-containing serum inhibits corticosterone-induced apoptosis in PC12 cells by downregulating[Ca2+]i overload
Xiaoyaosan-containing serum inhibits corticosterone-induced apoptosis in PC12 cells by downregulating[Ca2+]i overload
摘要
Abstract
Apoptosis in hippocampal neurons is an important contributor to depression.[Ca2+]i of hippocampal neurons may raise rapidly with increased cortioosterone concentrations,leading to apoptosis.The anti-depressant effects of Xiaoyaosan(Free and Easy Wanderer's Powder)may inhibit apoptosis by down regulating the[Ca2+]i overload.PC12 cells were containing with corticosterone to simulate neuronal injury,dosed with Xiaoyaosan-containing serum,and the following parameters were measured: apoptosis,[Ca2+]i overload,and changes in mitochondrial membrane potential(Δψm)and bioenergetics.High-dose Xiaoyaosan-containing serum(the dose of intragastric administration was 2.633 g herb/mL)not only inhibited morphological injury and cell apoptosis,but also suppressed[Ca2+]i overload induced by corticosterone.Xiaoyaosan also blocked the decrease in mitochondrial membrane potential and adenosine triphosphate levels induced by corticosterone.Xiaoyaosan dose-dependently suppresses corticosterone-induced apoptosis,potentially via inhibiting[Ca2+]i overload,and prevents impaired mitochondrial bioenergetics by maintaining mitochondrial membrane potential and adenosine triphosphate production,providing a putative mechanism for its antidepressant effects.关键词
Xiaoyaosan/corticosterone/apoptosis/PC12 cells/[Ca2+]i overload/neural regenerationKey words
Xiaoyaosan/corticosterone/apoptosis/PC12 cells/[Ca2+]i overload/neural regeneration分类
医药卫生引用本文复制引用
Lin Li,Xuanhong Zhang,Tingting Yan,Qian Li,Shukao Liu,Lili Wu,Can Yan,Hongmei Tan..Xiaoyaosan-containing serum inhibits corticosterone-induced apoptosis in PC12 cells by downregulating[Ca2+]i overload[J].中国神经再生研究（英文版）,2010,5(18):1396-1402,7.基金项目
This work was supported by the Ministry of Education of the People’s Republic of China, No. NCET-05-0750, and Guangdong Provincial Natural Science Research Team (Innovative research on traditional Chinese medicine theory of "Gan taking charge of dispersion" in prevention and treatment of damage caused by psychological stress), No. 5200491. （Innovative research on traditional Chinese medicine theory of "Gan taking charge of dispersion" in prevention and treatment of damage caused by psychological stress）
