中国组织工程研究与临床康复2010，Vol.14Issue(32)：6077-6080,4.DOI:10.3969/j.issn.1673-8225.2010.32.042
激光调控内源性神经干细胞的增殖分化及脑功能重建
Proliferation and differentiation of endogenous neural stem cells and brain functional reconstruction following laser irradiation
摘要
Abstract
BACKGROUND: Numerous studies have shown that the increase of reproductive activity and self-repair capacity of brain endogenous neural cells might be a valuable method to treat ischemia-hypoxia brain damage. OBJECTIVE: To study the effects of He-Ne laser irradiation on the proliferation and differentiation of endogenous neural stem cells and brain functional reconstruction in newborn rats with hypoxia-ischemia brain damage.METHODS: Newborn rats aged 7 days were prepared for hypoxia-ischemia brain damage models. From the second day of model induction, rats in the laser treatment group were subjected to He-Ne laser irradiation. Acupuncture point included Baihui (DU20) on the median parietal bone, and Dazhui (GV14) between the C7 and T1 and the median back. After the second course, the learning and memory ability of rats were tested by Y-type maze test. Then brain hippocampal sections were made and underwent immunohistochemistry for nestin and microtubule-associated protein-2.RESULTS AND CONCLUSION: In the laser treatment group, the ability of learning and memory were obviously higher than those in the model group (P < 0.05), however, compared with the sham-operated group, the difference was not obviously (P > 0.05). Compared with the sham-operated group, nestin expression in the dentate gyrus was significantly increased in the model and laser treatment groups (P < 0.05), and the increased range was greater in the laser treatment group compared with the model group (P < 0.05). Microtubule-associated protein-2 expression was widely distributed in the cerebral cortex, and darkly stained brown dendrite presented with radiation-shape. Neurons in the hippocampal pyramid and dentate gyrus granular cell layer arranged regularly. Positively stained dendrite presented branch-shape and distributed in the molecular layer. No significant difference was determined between the sham-operated and laser treatment groups. But the microtubule-associated protein-2 expression was significantly weakened in the model group. He-Ne laser irradiation can promote proliferation of endogenous neural stem cells in neonatal rats with hypoxia-ischemia brain damage, induce its differentiation into neurons, thus, achieves reconstruction of learning and memory functions.关键词
氦氖激光/学习记忆/缺血缺氧/神经干细胞/新生大鼠分类
医药卫生引用本文复制引用
张伟宏,李宛青,王金国,刘桂萍,杨华山,王璐璐..激光调控内源性神经干细胞的增殖分化及脑功能重建[J].中国组织工程研究与临床康复,2010,14(32):6077-6080,4.基金项目
the Tackle Key Program in Science and Technology of Science and Technology Commission of Henan Province,No.102102310130 （）
the Scientific and Technological Tackle Key Program of Science and Technology Bureau of Zhengzhou,No.10PTGS484-5 河南省科技厅科技攻关项目(102102310130) （102102310130）
郑州市科技局科技攻关支持项目(10PTGS484-5). （10PTGS484-5）
