首页|期刊导航|中华医学杂志（英文版）|Increased stromal-cell-derived factor 1 enhances the homing of bone marrow derived mesenchymal stem cells in dilated cardiomyopathy in rats
Increased stromal-cell-derived factor 1 enhances the homing of bone marrow derived mesenchymal stem cells in dilated cardiomyopathy in rats
ZHOU Yan-li Michael Fu ZHANG Hai-feng LI Xin-li DI Ruo-min YAO Wen-ming LI Dian-fu FENG Jian-lin HUANG Jun CAO Ke-jiang
中华医学杂志（英文版）2010，Vol.123Issue(22)：3282-3287,6.
中华医学杂志（英文版）2010，Vol.123Issue(22)：3282-3287,6.DOI:10.3760/cma.j.issn.0366-6999.2010.22.021
Increased stromal-cell-derived factor 1 enhances the homing of bone marrow derived mesenchymal stem cells in dilated cardiomyopathy in rats
Increased stromal-cell-derived factor 1 enhances the homing of bone marrow derived mesenchymal stem cells in dilated cardiomyopathy in rats
摘要
Abstract
Background Stem cell transplantation has been shown to have beneficial effects on dilated cardiomyopathy. However,mechanism for stem cell homing to cardiac tissue in dilated cardiomyopathy has not yet been elucidated.Methods Mesenchymal stem cells were obtained from rat bone marrow, expanded in vitro, and labeled with 99mTc.Cardiomyopathy model was induced by doxorubicin in rats. 99mTc labeled cells were infused into the left ventricles in cardiomyopathy and control rats. Sixteen hours after injection, animals were sacrificed and different tissues were harvested to measure specific radioactivity. By use of real-time polymerase chain reaction and immunohistochemistry,Mrna and protein expressions for stromal-cell-derived factor 1 in cardiac tissue were measured.Results Labeling efficiency of mesenchymal stem cells was (70.0±11.2)%. Sixteen hours after mesenchymal stem cell transplantation, the heart-to-muscle radioactivity ratio was increased significantly in cardiomyopathy hearts as compared to control hearts. Both Mrna and rotein expressions of stromal-cell-derived factor 1 were up-regulated in cardiomyopathy hearts as compared with control hearts.Conclusion In dilated cardiomyopathy induced by doxorubicin up-regulated expression of stromal-cell-derived factor 1in heart may induce mesenchymal stem cells home to the heart.关键词
mesenchymal stem cells/stromal-cell-derived factor 1/cardiomyopathyKey words
mesenchymal stem cells/stromal-cell-derived factor 1/cardiomyopathy分类
医药卫生引用本文复制引用
ZHOU Yan-li,Michael Fu,ZHANG Hai-feng,LI Xin-li,DI Ruo-min,YAO Wen-ming,LI Dian-fu,FENG Jian-lin,HUANG Jun,CAO Ke-jiang..Increased stromal-cell-derived factor 1 enhances the homing of bone marrow derived mesenchymal stem cells in dilated cardiomyopathy in rats[J].中华医学杂志（英文版）,2010,123(22):3282-3287,6.基金项目
This study was supported by the National Natural Science Foundation of China (No. 30900603), Specialized Research Fund for the Doctoral Program of Higher Education (No.20093234110008 and No. 20093234120008), and Project Supported by Department of Health of Jiangsu Province(No.ZX07 200902) （No. 30900603）
