中德临床肿瘤学杂志（英文版）2010，Vol.9Issue(12)：692-696,5.DOI:10.1007/s10330-010-0717-z
siRNA沉默STAT5对食管癌EC9706细胞增殖、凋亡和侵袭的影响
The effect of STAT5 silenced by siRNA on proliferation, apoptosis and invasion of esophageal carcinoma cell line EC9706
Daoxin Jin 1Wenqiao Zang 2Tao Wang 1Min Li 3Jieru Wan 4Guoqiang Zhao5
作者信息
- 1. Department of Microbiology and Immunology, College of Basic Medical Science, Zhengzhou University, Zhengzhou 450001, China
- 2. Luohe Medical College, Luohe 462002, China
- 3. Department of Hemato-tumor, The First Affiliated Hospital, College of TCM of Henan, Zhengzhou 450000, China
- 4. Grade 2007, Department of Clinical Laboratory of Science, Tianjin Medical University, Tianjin 300070, China
- 折叠
摘要
Abstract
Objective: The aim of the study was to explore the effect of STAT5 silenced by siRNA on proliferation, apoptosis and invasion of esophageal carcinoma cell line EC9706. Methods: The siRNA vectors aiming to STAT5 gene were con-structed. STAT5 siRNA was transfected into EC9706 cells by LipofectamineTM 2000. Changes of STAT5, Bcl-2 and Cyclin D1 were analyzed by Western blot and RT-PCR. Effect of STAT5 siRNA on EC9706 cells proliferation was determined by MTT.Effects of STAT5 siRNA on EC9706 cells cycle and apoptosis were detected by the flow cytometry. Boyden chamber was used to evaluate the invasion and metastasis capabilities of EC9706 cells. Results: The double strands oligonucleotide of siRNA aiming to STAT5 was successfully cloned into the pRNAT-U6.1 vector, and the target sequence was coincide with the design. RT-PCR and Western blotting detection demonstrated that the expression levels of STAT5, Bcl-2 and Cyclin D1 genes were obviously decreased in EC9706 cells transfected with STAT5 targeting siRNA expression vectors. STAT5 siRNA could suppress the proliferation of EC9706 cells. The proportions of S and G2/M periods frequency were significantly decreased (P<0.05), and the proportion of G0/G1 period frequency was significantly increased (P<0.05). The average amount of cells penetrating Matrigel was significantly decreased (P<0.05). Silencing the STAT5 induced the apoptosis of esophageal carci-noma cell line EC9706 (P<0.01). Conclusion: STAT5 silenced by siRNA of esophageal carcinoma call line EC9706 could induce the apoptosis. And it could suppress the proliferation, invasion and metastasis of tumor cells.关键词
STAT5/siRNA/cell proliferation/cell cycle/cell apoptosisKey words
STAT5/siRNA/cell proliferation/cell cycle/cell apoptosis分类
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Daoxin Jin,Wenqiao Zang,Tao Wang,Min Li,Jieru Wan,Guoqiang Zhao..siRNA沉默STAT5对食管癌EC9706细胞增殖、凋亡和侵袭的影响[J].中德临床肿瘤学杂志（英文版）,2010,9(12):692-696,5.
