首页|期刊导航|中国神经再生研究（英文版）|Inhibitory effects of HS014 on glutamate release in astrocytes chronically treated with morphine
中国神经再生研究（英文版）2010，Vol.5Issue(19)：1491-1494,4.DOI:10.3969/j.issn.1673-5374.2010.19.009
Inhibitory effects of HS014 on glutamate release in astrocytes chronically treated with morphine
Inhibitory effects of HS014 on glutamate release in astrocytes chronically treated with morphine
摘要
Abstract
Previous studies have confirmed that the number of glial fibrillary acidic protein-positive cells significantly increases during morphine tolerance.However,morphine tolerance is reversed with melanocortin receptor antagonists,and analgesic action is enhanced accordingly.However,these mechanisms remain unclear.In the present study,following addition of morphine to Wistar rat spinal cord astrocytes,glutamate levels in the supematant significantly increased(P<0.05).At 30-120minutes following addition of intervention agent to spinal cord astrocytes,naloxone significantly increased glutamate release in morphine-tolerant model cells(P < 0.05),while melanocortin receptor antagonist HS014 decreased glutamate release(P < 0.05).Additional naloxone and HS014 to astrocytes significantly decreased glutamate release compared with additional naloxone alone(P < 0.01).Results from the present study demonstrated that glutamate release was increased in spinal cord astrocytes co-cultured with morphine.Naloxone increased glutamate release,and HS014 reduced glutamate release.关键词
HS014/naloxone/astrocytes/morphine/glutamate/high performance liquid chromatography-tandem quadrupole mass spectrometryKey words
HS014/naloxone/astrocytes/morphine/glutamate/high performance liquid chromatography-tandem quadrupole mass spectrometry分类
医药卫生引用本文复制引用
Haichen Chu,Zejun Niu,Zhao Yang,Xuefeng Zhang..Inhibitory effects of HS014 on glutamate release in astrocytes chronically treated with morphine[J].中国神经再生研究（英文版）,2010,5(19):1491-1494,4.基金项目
This study was supported by the Natural Science Foundation of Shandong Province,No.Y2006C11. （）
