中国组织工程研究与临床康复2010，Vol.14Issue(41)：7770-7774,5.DOI:10.3969/j.issn.1673-8225.2010.41.041
汉防己甲素干预急性损伤脊髓神经元凋亡及bcl-2和bax表达:与甲基强的松龙的比较
Effects of tetrandrine on neuronal apoptosis,bcl-2 and bax expressions following acute spinal cord injury In comparison with methylprednisolone
摘要
Abstract
BACKGROUND:Studies have demonstrated that tetrandrine has protection on acute spinal cord injury,but the specific mechanism remains poorly understood.OBJECTIVE:To study the protection of tetrandrine on rat acute spinal cord injury and to study its mechanism from apoptosis pathway.METHODS:A total of 100 rats were randomly divided into 4 groups.All rats were prepared for spinal cord injury models using modified Allen method except that in the sham-surgery group.Methylprednisolone and tetrandrine was injected into rats in the methylprednisolone and tetrandrine groups by tail intravenous injection prior to and at 24,48 hours after model preparation.The same volume of physiological saline was injected in the sham-surgery and model groups.Basso-BeatUe-Bresnahan(BBB score)was recorded at 8 hours,1,3,7 and 14 days after model preparation.The morphological changes of spinal cord injury sites were observed by hematoxylin-eosin staining and the expressions of bcl-2 and bax were determined by immunohistochemistry.RESULTS AND CONCLUSION:The BBB score of methylpradnisolone and tetrandrine groups were significantly higher than that model group at 7 and 14 days(P<0.05),but there were no significant difference between the methylprednisolone group and tetrandrine group(P>0.05).Hematoxylin-eosin staining showed that the spinal cord injured severely at 3-7 days,the injury degree in the methylpradnisolone group and tetrandrine group was slighter than that of the model group,with smaller bax expression and greater bcl-2 expression(P<0.01).The findings demonstrated that,tetrandrine is able to protect neurons from apoptosis and promote the nerve function recovery by inhibiting the expression of Bax and promoting the expression of Bcl-2.Its effect is not inferior to methylprednisolone.关键词
汉防己甲素/急性脊髓损伤/凋亡/bcl-2/bax/甲基强的松龙分类
医药卫生引用本文复制引用
罗春山,田晓滨,汪雷,李波,彭智,周焯家,简月奎,赵伟峰..汉防己甲素干预急性损伤脊髓神经元凋亡及bcl-2和bax表达:与甲基强的松龙的比较[J].中国组织工程研究与临床康复,2010,14(41):7770-7774,5.基金项目
贵州省省长资金:黔省专合字[2005]27号 （）
贵州省卫生厅基金:黔卫发[2003]150文件.the Governor Foundation of Guizhou Province,No.[2005]27 （）
the Foundation of Health Department of Guizhou Province,No.[2003]150 （）
