中国组织工程研究与临床康复2010，Vol.14Issue(42)：7971-7975,5.DOI:10.3969/j.issn.1673-8225.2010.42.044
纳米晶羟基磷灰石胶原复合骨髓间充质干细胞修复骨缺损
Nano-hydroxyapatite/collagen composite co-cultured with bone marrow-derived mesenchymal stem cells for repair of bone defects
赵基栋 1钱寒光 1苗宗宁 1祝建中 1彭玮1
作者信息
- 1. 无锡市第三人民医院修复重建外科,江苏省无锡市,214041
- 折叠
摘要
Abstract
BACKGROUND: There have been no effective means for clinical treatment of large regions of bone defects.Nano-hydroxyapatite/collagen(nHAC)composite would provide a new pathway for repair of bone defects owing to its similar structure to natural skeleton and better biocompatibility.OBJECTIVE: To investigate the role of nHAC composite co-cultured with bone marrow-derived mesenchymal stem cells(BMSCs)in repair of bone defects.METHODS: Following isolation and culture,human BMSCs were co-cultured with nHAC composite.Gross observation,histological analysis,and electron microscope observation were performed to analyze osteogenesis for repair of bone defects in the clinic.RESULTS AND CONCLUSION: Human nHAC could greatly proliferate in vitro.X-ray photography revealed that bone defects well healed after implantation of nHAC/BMSCs composite.These findings indicate that BMSCs exhibit osteogenic potential and nHAC is a satisfactory scaffold material for construction of tissue-engineered bone.关键词
骨髓间充质干细胞/羟基磷灰石/纳米材料/组织工程/骨分类
基础医学引用本文复制引用
赵基栋,钱寒光,苗宗宁,祝建中,彭玮..纳米晶羟基磷灰石胶原复合骨髓间充质干细胞修复骨缺损[J].中国组织工程研究与临床康复,2010,14(42):7971-7975,5.
