首页|期刊导航|中华医学杂志（英文版）|Heme oxygenase-1 regulates the major route involved in formation of immune hepatic fibrosis in rats
中华医学杂志（英文版）2010，Vol.123Issue(22)：3304-3308,5.DOI:10.3760/cma.j.issn.0366-6999.2010.22.025
Heme oxygenase-1 regulates the major route involved in formation of immune hepatic fibrosis in rats
Heme oxygenase-1 regulates the major route involved in formation of immune hepatic fibrosis in rats
摘要
Abstract
Background Heme oxygenase (HO) plays roles in some liver diseases, but what it does in immune liver fibrosis is rarely reported. We investigated the regulation mechanisms of HO-1 in rat immune liver fibrosis to find routes for intervention.Methods Male Sprague-Dawley rats were randomly divided into control group (N, n=12), fibrosis group (F, n=20),cobalt protoporphyrin (CoPP) inducing group (Co, n=20) and zinc protoporphyrin (ZnPP) inhibiting group (Zn, n=20). In groups F, Co and Zn, immune liver fibrosis was established with human serum albumin. At the attacked stage, CoPP (5mg/kg) and ZnPP (5 mg/kg) were intraperitoneally injected in groups Co and Zn, respectively. After establishment of rat models, the numbers of rats reduced to 11, 15, 17 and 12 in groups N, F, Co and Zn respectively, because of death during the process. HO-1 in liver was detected by Westem blotting and immunohistochemistry. The indexes of fibrosis were assessed by radioimmunoassay. Concentrations of serum transforming growth factor-β1 (TGF-β1), and tissue inhibitor of metalloproteinses (TIMP-1) were detected using enzyme-linked immunosorbent assay. Hepatic stellate cell (HSC) and proliferation degree of fibrosis were assessed by pathological examination. Data analysis was performed by SPSS 10.0software.Results The expression of HO-1 in group F was significantly higher than that in group N, but lower than that in group Co (P ＜0.05); while that in group Zn was lower than in group F (P ＜0.05), but still higher than that in group N (P ＜0.01).Compared with group N, liver functional and liver fibrosis indicators were increased in group F (P ＜0.01), while comparing to group F, they were decreased in group Co (P ＜0.05) and increased in group Zn (P ＜0.05). CoPP reduced the extent of hepatocellular injury and hepatic fibrosis in comparison with group F (P ＜0.01), being the opposite effect of ZnPP (P＜0.01). HSC was observed using indirect method and the result showed that the number of HSC in group F increased more than that in groups N and Co, while much less than in group Zn. The concentration of TGF-β1 decreased when HO-1 expressed increasingly (group Co: (3.5±1.0) ng/ml, group F: (7.8±1.3) ng/ml, P ＜0.01) and enhanced (group Zn:(9.6±13.6) ng/ml) when HO-1 presented less (P ＜0.01). The concentrations of TIMP-1 were (151.1±32.0), (472.0±34.8),(232.3±41.3) and (533.2±37.2) ng/g liver wet weight in groups N, F, Co, and Zn, respectively. It was reduced in group Co (P＜0.01) and increased in group Zn compared with group F (P ＜0.05).Conclusions Inducing HO-1 expression appropriately may lighten hepatic fibrosis, and in contrast, inhibiting it strengthens the lesion. HO-1 interferes with the main ways to form liver fibrosis.关键词
heme oxygenase-1/immune fibrosis/ratsKey words
heme oxygenase-1/immune fibrosis/rats分类
医药卫生引用本文复制引用
DUAN Zhi-jun,YANG Dong,WANG Fei,SUN Ying-jie,SUN Xiao-yu,ZHENG Miao-lei..Heme oxygenase-1 regulates the major route involved in formation of immune hepatic fibrosis in rats[J].中华医学杂志（英文版）,2010,123(22):3304-3308,5.基金项目
This study was supported by grants from the National Natural Science Foundation of China (No. 2005-30570515) （No. 2005-30570515）
the Educational Department Project of Liaoning Province (No.2004-F063) （No.2004-F063）
the Natural Science Fund Projects of Liaoning Province (No.2006-1058) （No.2006-1058）
Science and Technology Project of Dalian City (No.2002-B3NS137) （No.2002-B3NS137）
and the Project Sponsored by the Scientific Research Foundation for Returned Overseas Chinese Scholars, State Education Ministry (No.2005-546). （No.2005-546）
