首页|期刊导航|中华医学杂志（英文版）|Sequence of fat partitioning and its relationship with whole body insulin resistance
中华医学杂志（英文版）2010，Vol.123Issue(24)：3605-3611,7.DOI:10.3760/cma.j.issn.0366-6999.2010.24.019
Sequence of fat partitioning and its relationship with whole body insulin resistance
Sequence of fat partitioning and its relationship with whole body insulin resistance
摘要
Abstract
Background Currently it is unclear whether lipid accumulation occurs in a particular sequence and its relationship with whole body insulin resistance (IR). This study aimed to answer this question.Methods Male Sprague-Dawley (SD) rats were fed on a normal or a high-fat diet for 20 weeks. Serum triglycerides (TG), serum free fatty acids (FFA), fasting plasma glucose (FPG), and liver and skeletal muscle TG were measured. The glucose infusion rate (GIR) and mRNA levels of acetyl-CoA carboxylase (ACC) and carnitine palmitoyltransferase-1 (CPT-1) in the liver and skeletal muscle were determined at different stages.Results Compared with rats fed on the normal diet, serum FFA was not significantly increased in rats fed on the high-fat diet until 20 weeks. In contrast, liver TG was significantly increased by the high-fat diet by four weeks (20-fold; P ＜0.01),and remained elevated until the end of the study. However, skeletal muscle TG was not significantly increased by the high-fat diet until 20 weeks (10.6-fold; P＜0.01), and neither was the FPG. The GIR was significantly reduced (1.6-fold; P ＜0.01) by the high-fat diet after 8 weeks. The mRNA levels of ACC gradually increased over time and CPT-1 decreased over time, in both the liver and skeletal muscle in rats fed the high-fat diet.Conclusions Lipid accumulation in the liver occurs earlier than lipid accumulation in the skeletal muscle. Fatty liver may be one of the early markers of whole body IR. Changes in the gene expression levels of ACC and CPT-1 may have important roles in the process of IR development.关键词
body fat distribution/insulin resistance/dietary fats/Sprague-Dawley ratKey words
body fat distribution/insulin resistance/dietary fats/Sprague-Dawley rat分类
医药卫生引用本文复制引用
BAI Xiu-ping,LI Hong-liang,YANG Wen-ying,XIAO Jian-zhong,WANG Bing,LOU Da-jun,DU Rui-qin..Sequence of fat partitioning and its relationship with whole body insulin resistance[J].中华医学杂志（英文版）,2010,123(24):3605-3611,7.基金项目
This research was partly supported by a grant from the National Natural Science Foundation of China (No. 30670995). （No. 30670995）
