环境与职业医学2011，Vol.28Issue(1)：25-28,4.
百草枯对体外培养小鼠胚胎神经干细胞增殖的影响
Effects of Paraquat on the Proliferation Viability of Mouse Embryo Neural Stem Cells in Vitro
摘要
Abstract
[Objective]To isolate, cultivate and identify the neural stem cells( NSCs )of mouse embryo in vitro and study the effect of paraquat( PQ )on their proliferation viability.[Methods]Cerebral cortexes were isolated from embryonic ( E 10.5 d ) mice.The cells were identified as neural stem cells through immunocytochemistry assey with antibodies to neural stem cells( NSCs ) specific protein and morphological observation.After treatment with 0.00, 0.10, 1.00, 10.00, 100.00, 1000.00 μmol/L PQ for 24h,the viability of the NSCs was measured by Alamar Blue assay.After treatment with 0.00, 0.10, 1.00, 10.00, 50.00, 100.00 μmol/L PQ for 24h, the reactive oxidative spieces( ROS )production of the NSCs was measured by DCFH-DA assay.[Results]The result of immunocytochemistry assey showed that nestin which is the specific protein of NSCs was expressed in the majority of the cells.Alamar Blue assey reveals that the cell viability was reduced significantly in the groups of PQ ≥ 100.00 μmol/L compared with the control group ( P ＜ 0.05 ).With the increase concentration of PQ, the cell viability decreased accordingly ( P ＜ 0.05 ).DCFH-DA result showed that the ROS production increased significantly in the groups received PQ ≥ 10.00 μmol/L compared with the control group( P＜0.05 ).Concentration with the increase of PQ, the ROS production increased accordingly( P＜0.05 ).[Conclusion]Primary culture of NSCs in vitro was established successfully.PQ contributed a dose-dependent relationship on suppression of the proliferation and raise of ROS level of NSCs.关键词
神经干细胞/百草枯/增殖Key words
neural stem cells/ paraquat/ proliferation分类
预防医学引用本文复制引用
吕文,常秀丽,吴庆,周志俊..百草枯对体外培养小鼠胚胎神经干细胞增殖的影响[J].环境与职业医学,2011,28(1):25-28,4.基金项目
国家科技支撑计划(编号:2006ABI06B01),上海市自然科学基金(编号:10ZR1401700),上海市公共卫生重点学科建设项目(编号:08GWZX303),上海市学科带头人培养项目(编号:08GWD20) （编号:2006ABI06B01）
