浙江医学2011，Vol.33Issue(1)：54-57,4.
应用MOG-TrxA建立小鼠EAE动物模型的新方法
Mouse experimental autoimmune encephalomyelitis model induced by MOG-TrxA immunization
张国勇 1张旭 2厉向2
作者信息
- 1. 325000,温州市第二人民医院神经内科
- 2. 温州医学院附属第一医院神经内科
- 折叠
摘要
Abstract
Objective To establish mouse experimental autoimmune encephalomyelitis (EAE) model induced by MOG-TrxA immunization of C57BL/6(H-2b) mice engineering. Methods MOG-TrxA and TrxA was expressed in E. coli BL21 by transfection of recombinant plasmids pET32(a)-MOG-TrxA and pET32(a)-TrxA respectively, The purified proteins were applied to immunize C57BL/6 (H-2b) mice to induce EAE (MOG-TrxA) and control (TrxA). The mice immunized with spinal cord homogenate (SCH) as the positive control. The clinical scores curve, weight variation curve, HE staining and Klü ver-Barrera staining scores were observed to evaluate quality of EAE model. Results There were significant differences in clinical scores, weight changes, HE staining scores and Klüver-Barrera staining scores between MOG-TrxA group and TrxA group (P ＜0.05), while there were no significant differences between MOG-TrxA group and SCH group (P ＞0.05). Conclusion The EAE animal model has been successful established by immunization of gene-engineered MOG-TrxA in C57BL/6(H-2b) mice.关键词
实验性自身免疫性脑脊髓炎/多发性硬化/少突胶质细胞糖蛋白引用本文复制引用
张国勇,张旭,厉向..应用MOG-TrxA建立小鼠EAE动物模型的新方法[J].浙江医学,2011,33(1):54-57,4.
