中山大学学报（医学科学版）2011，Vol.32Issue(1)：85-89,5.
复发性流产与夫妻双方绒毛血管内皮生长因子基因多态性的关系
Association of Recurrent Spontaneous Abortion with Parental and Chorionic Villi' s Vascular Endothelial Growth Factor Gene Polymorphism in Southern Chinese Population
摘要
Abstract
[Objective]To explore the association of parental and chorionic villi's polymorphisms of vascular endothelial growth factor (VEGF) gene (-1154G＞A) with recurrent spontaneous abortion (RSA) in southern Chinese population. [Methods] DNA was extracted from blood samples and chorionic villi, and analyzed by allele-specific PCR. A total of 271 couples with a history of idiopathic RSA, together with 250 age-matched control women and 200 age-matched control men were recruited. The specimens of chorionic villi were collected from 60 cases of spontaneous abortion at early stage and 60 normal early pregnancies. Allele-specific amplification Polymerase chain reaction (ASA-PCR) was applied to analyze the -1154G ＞ A genotype. [Results]Statistically significant differences in the GA, AA, and GG genotype frequencies were observed between female patients with RSA and the controls (P=0.012). The risk of RSA was higher in the carriers of the A allele than in women carrying the G allele (P = 0.003, OR = 1.562,95％ CI = 1.157-2.109). The frequencies of three genotypes of VEGF -1154G ＞ A polymorphism had no difference between male patients or chorionic villi with RSA and the controls (P = 0.837 and 0.708). The allelic frequencies were similar between male patients or chorionic villi with RSA and the controls (P = 0.572 and 0.757). [Conclusion]VEGF-1154G/A polymorphism of female is associated with susceptibility to RSA; carrying A allele may increase the risk of RSA in southern Chinese women, whereas VEGF-1154G/A polymorphism of male and chorionic villi are not associated with RSA. These results may imply that maternal VEGF-1154G/A polymorphism may be more closely related to RSA than embryo-fetal VEGF-1154G/A polymorphism.关键词
血管内皮生长因子/基因多态性/复发性自然流产分类
医药卫生引用本文复制引用
李珠玉,游泽山,苏秀梅,张秀明,李再仪,吴再归,江丽..复发性流产与夫妻双方绒毛血管内皮生长因子基因多态性的关系[J].中山大学学报（医学科学版）,2011,32(1):85-89,5.基金项目
广东省科技计划项目(2008B030301096) （2008B030301096）
广州市黄埔区科技计划项目(0931) （0931）
