中山大学学报（医学科学版）2011，Vol.32Issue(1)：104-108,5.
荧光标记线粒体DNA SNPs检测体系建立及单倍型频率调查
Establishment of Typing for Mitochondrial DNA SNPs and Investigation of Haplotype Frequency
摘要
Abstract
[Objective]To develop the method typing SNPs of mitochondrial DNA with fluorescence labeling and apply to the forensic science practice.[Methods]Eighteen SNPs selected from mtDNA (16 SNPs in the coding region and 2 SNPs in the control region) were studied the rules of of primers’ design multiplex system and multiplex sequencing system then carried out capillary electrophoresis.[Results]The 18-plex mitochondrial DNA SNPs minisequencing reaction was developed and the data of SNP haplotype frequency in Guangdong Han population was obtained.[Conclusion]The multiplex amplication of mitochondrial DNA SNPs was established and showed that the system has a favorable prospective in the forensic science.关键词
单核苷酸多态性(SNPs)/线粒体/复合扩增/微测序/毛细管电泳分类
医药卫生引用本文复制引用
何琼,刘长晖,王慧君,刘超..荧光标记线粒体DNA SNPs检测体系建立及单倍型频率调查[J].中山大学学报（医学科学版）,2011,32(1):104-108,5.基金项目
国家十一五科技支撑计划(2006BAK07B01) （2006BAK07B01）
公安部重点科技攻关项目(20034421401) （20034421401）
