解放军医学杂志2011，Vol.36Issue(2)：161-163,3.
KAI1基因外源性过表达对胆管癌细胞层黏素受体的影响
Influence of exogenous overexpression of KAI1 on the expression of laminin receptor in human cholangiocarcinoma cell line
邓小明 1王曙光 2张丰深 3孙海 3杨星 3杨琳3
作者信息
- 1. 解放军324医院肝胆外科
- 2. 400038,重庆,第三军医大学西南医院全军肝胆外科研究所
- 3. 400020,重庆,解放军324医院肝胆外科
- 折叠
摘要
Abstract
Objective To investigate the effects of exogenous overexpression of KAI1 on laminin receptor (LNR) in human cholangiocarcinoma QBC939 cell line Methods Human cholangiocarcinoma QBC939 cells were cultured in vitro and divided into two groups: control group (transfecting empty vector, pIRES2-EGFP) and experimental group (transfecting recombinant eukaryotic vector,pIRES2-EGFP-KAI1). The pIRES2 EGFP and pIRES2-EGFP-KAI1 were transfected into QBC939 cells line with Lipofectamine 2000.After being screened with G418, the mRNA was then extracted and detected by reverse transcription-polymerase chain reaction (RT-PCR) analysis using KAI1 and LNR-specific primers, and an aliquot of protein from each sample was subjected to Western blotting analysis using KAI1 and LNR antibodies. The protein expressions of KAI1 and LNR in two groups were also measured by immunocytochemistry. Results The mRNA and protein levels of KAI1 were significantly higher in experimental group (0. 49±0. 07 and 1. 06±0. 05) than in control group (0. 22±0. 02 and 0. 59±0. 02, P＜0. 01). On the contrary, The mRNA and protein levels of LNR were significantly lower in experimental group (0. 38±0. 04 and 0. 29±0. 03) than in control group (0. 73±0. 05 and 0. 68±0. 02, P＜0. 05). As Compared with the control group, the staining in cytoplasm of KAI1 was intensified and LNR diminished after pIRES2-EGFP-KAI1 plasmid was transfected.Conclusion Exogenous overexpression of KAI1 may down-regulate the LNR mRNA and protein expression in QBC939 cells in vitro.关键词
KAI1/层黏素受体/基因表达/胆管癌细胞Key words
KAI1/ laminin receptor/ gene expression/ biliary carcinoma cell分类
医药卫生引用本文复制引用
邓小明,王曙光,张丰深,孙海,杨星,杨琳..KAI1基因外源性过表达对胆管癌细胞层黏素受体的影响[J].解放军医学杂志,2011,36(2):161-163,3.
