解放军医学杂志2011，Vol.36Issue(2)：145-148,4.
C-fos和增殖细胞核抗原在人胎小肠组织中的表达及意义
Expression and significance of C-fos and proliferating cell nuclear antigen in the small intestinal tissue of human fetus
摘要
Abstract
Objective To explore the expression rule of proliferating cell nuclear antigen (PCNA), C-fos proteins and apoptosis genes in the small intestinal tissue of human fetus. Methods At the second-to fourth-month of gestation, the expressions of cell proliferation and apoptosis were observed in 16 specimens of human fetal small intestinal tissue by using the immunohistochemical methods and terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL). Results At the second to fourth month of gestation,all the PCNA and C-los proteins were positively expressed in the small intestinal tissues and cells of human fetus. With the increase in gestational period, the positive cell number and average internsity (Al) of PCNA protein increased gradually (P＜0. 01). The positive cell number of C-los protein increased first, and then decreased, while the Al of C-los protein stably increased in the small intestinal tissues and cells of human fetus (P＜0. 01). At the second to fourth month of gestation, TUNEL positive cells were seen to distribute in each layer of the small intestinal tissues of human fetus. With the increase of age, all the positive cell number and AI of TUNEL positive cells showed a tendency of decrease following increase in the small intestine of human fetus (P＜0. 01). Conclusions PCNA, C-los and apoptosis gene participate in adjusting the growth and development of the cells and tissues in the small intestine of human fetus In the third month of gestation, especially, proliferation and apoptosis are significantly increased in the small intestinal tissue of human fetus, which may be the key period of intestinal tissue development.关键词
小肠/增殖细胞核抗原/原癌基因蛋白质c-fos/胚胎/免疫组织化学/原位缺口末端标记Key words
small intestine/ proliferating cell nuclear antigen/ C-fos protein/ Human fetus/ Immunohistoehemistry/ terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL)分类
医药卫生引用本文复制引用
刘学红,张泳..C-fos和增殖细胞核抗原在人胎小肠组织中的表达及意义[J].解放军医学杂志,2011,36(2):145-148,4.基金项目
浙江省教育厅科研计划项目(Y200805569) （Y200805569）
绍兴文理学院重点资助项目(08LG1018) （08LG1018）
