解放军医学杂志2011，Vol.36Issue(2)：149-152,4.
Darbufelone对胃癌SGC-7901细胞裸鼠移植瘤生长的影响
Effects of darbufelone on the growth of tumor xenograft with SGC-7901 gastric cancer cells in nude mouse
摘要
Abstract
Objective To evaluate the effect of darbufelone, a5-1ipoxygenase/cyclooxygenase-2 (5-LOX/COX-2) dual inhibitor, on the growth of subcutaneous tumor xenograft of SGC-7901 gastric cancer cells and its mechanisr. Methods The xenograft model of human gastric carcinoma with SGC-7901 gastric cancer cells was reproduced in nude mice. Fourteen nude mice were randomly divided into the darbufelone group and the control group (7 each). The mice were consecutively given intagastric administration with 45mg/(kg d) darbufelone in darbufelone group, or NS in control group for 4 week. The tumor volume in each group was measured regularly, and the tumor growth inhibition was calculated. The xenograft, liver, kidneys, spleen and lungs of nude mice were observed pathologically after HE staining. The expressions of mRNA and protein of 5-LOX, COX-2, PCNA, Bcl-2 and caspase-3 in transplanted tumor were detected by RT-PCR and immunohistochemistry. Results Darbufelone showed significant inhibitory effects, with an inhibition ratio of 58. 42％, on the growth of tumor xenograft in vivo. The volume and weight of tumor in darbufelone group decreased significantly compared with that in control group (P＜0. 05). Darbufelone inhibited the growth of tumor xenograft with no adverse effect. Immunohistochemistry and RTPCR analyses showed that darbufelone effectively inhibited the expression of mRNA and protein of 5-LOX, COX-2, PCNA, Bcl-2, while increased the expression of caspase-3 compared with that in the control group (P＜0. 05). Conclusions Darbufelone can inhibit the growth of gastric carcinoma in vivo, the mechanism may be related to inhibition of growth of cancer cells and the induction of apoptosis.关键词
环氧化酶2抑制剂/脂氧合酶抑制剂/胃肿瘤/移植Key words
cyclooxygenase 2 inhibitors/ lipoxygenase inhibitors/ stomach neoplasms/ transplantation分类
医药卫生引用本文复制引用
徐晓萌,刘纯伦,谢黎..Darbufelone对胃癌SGC-7901细胞裸鼠移植瘤生长的影响[J].解放军医学杂志,2011,36(2):149-152,4.基金项目
重庆市卫生局资助课题(NO05-2-155) （NO05-2-155）
