农业环境科学学报2011，Vol.30Issue(1)：49-54,6.
柴油降解菌的筛选鉴定及降解特性研究
Screening and Identification of Diesel Fuel Degrading Microorganism
摘要
Abstract
The diesel fuel degrading bacteria used in this study was isolated from the aged oily sludge of Shengli Oil Field, and tentatively identified as A cinetobacter sp. through morphological and Biolog microplate analysis, as well as 16S rRNA sequencing. The strain, designated as WS14, was further characterized for its ability to degrade diesel fuel through determining the optical density(OD600), and the results of growth curves showed that the optimum condition was at pH 6.0～8.5, diesel concentration of 11.5％, and the inoculated temperature and time of 35℃ and 72 h, respectively. In addition, the WS14 strain had capacity to grow in 5％ salty and 30％ diesel containing medium. The WS14 strain was proposed a potential biodegradation for diesel oil and the biodegradation efficiencies varied as following 58.6％, 35.4％, 18.2％ for 1％, 5％ and 10％ diesel concentration, respectively. In addition, the chromatographic fingerprints indicated that the alkenes with less carbon numbers were preferentially degraded.关键词
柴油/微生物降解/筛选/菌种鉴定Key words
diesel fuel/ biodegradation/ screening/ strain identification分类
资源环境引用本文复制引用
王世杰,王翔,卢桂兰,汪群慧,李发生,郭观林..柴油降解菌的筛选鉴定及降解特性研究[J].农业环境科学学报,2011,30(1):49-54,6.基金项目
国际科技合作项目(2008DFR90550) （2008DFR90550）
全国土壤调查及污染防治专项(5120801) （5120801）
