中国药房2011，Vol.22Issue(2)：132-134,3.
大肠埃希菌和肺炎克雷伯菌产β-内酰胺酶的底物动力学研究
Study on the Kinetics of β-lactamase in Escherichia Coli and Klebsiella Pneumoniae
摘要
Abstract
OBJECTIVE: To study the enzyme kinetics ofβ-lactamase extracted from Escherichia coli and Klebsiella pneumoniae. METHODS: 8 strains of β-lactamases-producing E. coli and K. pneumoniae were collected from clinical specimens in our hospital in 2008. Antimicrobial susceptibility was tested by Kirby-Bauer method. Michaelis constant (Km) and maximal velocity (Vmax)were obtained by L-B Plot. RESULTS: 8 strains were all resistant to the β-lactam antibiotics and highly susceptible to carbapenem.The penlcillins, the first generation cephalosporins were hydrolyzed by all β-lactamase extracted from E. coli and K. pneumoniae.But the second and third generation cephalosporins were hydrolyzed by all β-lactamase extracted from E. coli and K. pneumonia in different degree. CONCLUSION: Carbapenem is the first choice for the treatment of infections induced by β-lactamases-producing strains. There are significant differences among hydrolysate profiles of β-lactamases produced by different β-lactamases-producing strains.关键词
β-内酰胺酶/酶动力学/β-内酰胺类抗生素分类
药学引用本文复制引用
李勇湧,黄文祥,朱卫民,张彬..大肠埃希菌和肺炎克雷伯菌产β-内酰胺酶的底物动力学研究[J].中国药房,2011,22(2):132-134,3.基金项目
重庆市自然科学基金资助项目(CSTC,2007BB5317) （CSTC,2007BB5317）
重庆市教委科学技术研究项目基金资助项目(KJ070308) （KJ070308）
