中国药房2011，Vol.22Issue(2)：134-136,3.
RP-HPLC法测定人血清中米氮平浓度
Concentration Determination of Mirtazapine in Human Serum by RP-HPLC
马金秋 1阎彦 1吴纪宁 1王娜1
作者信息
- 1. 河北秦皇岛市第一医院药学部,秦皇岛市,066000
- 折叠
摘要
Abstract
OBJECTIVE: To establish a method for concentration determination of mirtazapine in human serum.METHODS:RP-HPLC was adopted.An Intersil ODS-SP column was used with a mobile phase consisted of acetonitrile-0.01mol·L-1 ammonium acetate(30∶ 70).The flow rate was 1.0 mL·min-1 and fluorescence detection wavelength λcx at 290 nm and λcm at 350 nm.Metoprolol was used as internal standard.RESULTS: Mirtazapine showed a good linearity in range of 1.852～900 μg·L-1(r=0.999 1).The relative recovery and extraction recovery for mirtazapine were 94.8％～104.0％ and 97.8％～106.3％.The intra-day RSD was less than 13 ％ and inter-day RSD was less than 8％.The minimal detectable concentration was 1.852 μg· L-1.CONCLUSION: This method is sensitive, simple, rapid and accurate for clinical pharmacokinetic study and plasma concentration monitoring of mirtazapine.关键词
反相高效液相色谱法/米氮平/血清/浓度分类
医药卫生引用本文复制引用
马金秋,阎彦,吴纪宁,王娜..RP-HPLC法测定人血清中米氮平浓度[J].中国药房,2011,22(2):134-136,3.
