中国药房2011，Vol.22Issue(2)：130-131,2.
HPLC法测定人血浆中多西紫杉醇浓度
Determination of the Concentration of Docetaxel in Human Plasma by HPLC
摘要
Abstract
OBJECTIVE: To establish a method for the concentration determination of docetaxel in human plasma. METHODS: Using the external standard method, plasma samples were extracted with acetonitrile, shaken, centrifugated and filtrated.Kromasil C18(250 mm×4.6 mm, 5 μm)column was adopted with mobile phase consisted of acetonitrile at flow rate of 1.0 L·min-1.The detection wavelength was set at 230 nm. Column temperature was room temperature, sensitivity was 2.0AUFS and injection volume was 10 μL. RESULTS: The linear range of docetaxel was 0.167～3.333 μg·mL-1(r=0.995 2). The detection limit for docetaxel was 0.167 μg·mL-1. The extraction recovery was ranged from 84.7％ to 87.5％. The RSD of inter-day and intra-day were both lower than 10％. CONCLUSION: The method is simple, accurate and rapid for the plasma concentration monitoring of docetaxel.关键词
高效液相色谱法/血药浓度/多西紫杉醇分类
药学引用本文复制引用
陈瑶,赵银,刘青青,邹俊..HPLC法测定人血浆中多西紫杉醇浓度[J].中国药房,2011,22(2):130-131,2.基金项目
云南省社会发展科技计划(应用基础研究)项目(2009ZC171M) （应用基础研究）
