农业生物技术学报2011，Vol.19Issue(1)：122-127,6.DOI:10.3969/j.issn.1674-7968.2011.01.018
肌肉生长抑制素基因(MSTN)外显子1的多态性及其与边鸡生长性状的关联分析
Polymorphisms in Exon 1 of the Myostatin Gene (MSTN) and Its Relationship with Growth Traits in Bian Chicken
摘要
Abstract
In order to lay a foundation for Marker-assisted Selection (MAS) for Bian chicken's growth traits,single nucleotide polymorphisms (SNPs) of exon 1 of the myostatin (MSTN) gene were detected in Bian chicken (Gallus gallus) and three reference chicken (G.gallus) populations (the Jinghai, Youxi and Arbor Acre chickens) by PCR-SSCP.And the relationship with growth traits in the Bian chicken was analysed.Results showed that the Bian, Jinghai and Youxi chickens all displayed six genotypes (AA, AB, BB, BC, AC and CC), while only four genotypes (AB, BC, AC and CC) were detected in the Arbor Acre chicken.Sequencing revealed three mutations (G2 100A, G2 109A and C2 244G) of the MSTN gene.Chickens ofBB genotype had significantly higher body weight than those of AA genotype (P＜0.05) from 6 to 8 week of age and 12 week of age.Chickens ofBB genotype had significantly higher body weight than those of AA and AC genotypes at 14 week of age (P＜0.05).Chickens of BB genotype had significantly higher body weight than those of the other five genotypes from 16 to 18 week of age (P＜0.05).These results preliminarily show that the MSTN gene is either a major gene that influences the body weight traits of the Bian chicken or a molecular marker in close linkage with them.关键词
肌肉生长抑制素/边鸡/PCR-SSCP/生长性状Key words
Myostatin (MSTN)/ Bian chicken/ PCR-SSCP/ Growth traits引用本文复制引用
张跟喜,丁馥香,是燕萍,王金玉,张李俊,张丽,施会强..肌肉生长抑制素基因(MSTN)外显子1的多态性及其与边鸡生长性状的关联分析[J].农业生物技术学报,2011,19(1):122-127,6.基金项目
本研究由国家肉鸡产业技术体系(No.nycytx-42-G1-05)、山西省科技攻关(No.20100311001)、山西省自然基金项目(No.2009011043-2)和江苏省动物遗传繁育与分子设计重点实验室共同资助 （No.nycytx-42-G1-05）
