中国药房2011，Vol.22Issue(7)：616-618,3.
7种松柏科植物中莽草酸的含量测定
Content Determination of Shikimic Acid in 7 Kinds of Coniferous Plants
摘要
Abstract
OBJECTIVE: To establish a method for the content determination of shikimic acid from coniferous plants, and to confirm the best raw material for shikimic acid and collecting season. METHODS: Shikimic acid from needles and branches of 7 kinds of coniferous plants was determinated by HPLC. Diamonsil C18( 150 mm×4.6 mm,5 μm) was used, the mobile phase consisted of 0.1％ phosphorus solution-methanol (90:10),the flow rate was 1.0 mL·min-1, the detection wavelengh was set at 217 nm and the column temperature was 30 ℃. RESULTS: The linear range of shikimic acid was 4～40 μg·mL-1(r=0.999 6) with an average recovery of 97.9％ (RSD=1.43％, n=9). The highest contents of shikimic acid in needles and branches from coniferous plants both collected from Cedrus deodara were 3.93％ and 1.66％ respectively. The content of shikimic acid from pine needles of C. deodara in May was the highest 4.11％. CONCLUSION: The method is convenient, accurate and reliable for content determination of shikimic acid. The pine needles of C. deodara can be used as raw material of shikimic acid and April to June is the best collecting season.关键词
松柏科/松针/采集期/莽草酸/高效液相色谱法/含量测定分类
医药卫生引用本文复制引用
王东东,石晓峰,李冲,沈薇,刘东彦,范彬,马趣环..7种松柏科植物中莽草酸的含量测定[J].中国药房,2011,22(7):616-618,3.基金项目
甘肃省科技计划项目(0709TCYA022) （0709TCYA022）
甘肃省卫生厅科研计划项目(WST07-02) （WST07-02）
