癌变·畸变·突变2011，Vol.23Issue(1)：26-30,5.DOI:10.3969/j.issn.1004-616x.2011.01.007
黄曲霉毒素B1诱导的恶性转化肝细胞miRNA表达谱的变化
Differentially expressed miRNA profile of transformed hepatic cells induced by aflatoxin B1
摘要
Abstract
OBJECTIVE: To identify the differentially expressed miRNAs in malignantly transformed L02 cells(L02T) induced by aflatoxin B1 (AFB1), we performed miRNA microarray on both control cells(L02) and L02T cells. METHODS: L02T cells were obtained after exposure to aflatoxin B1 (AFB1) several times. The comparison of differential expression profiles between L02 and L02T cells was performed and analyzed. The differentially expressed miRNAs were then selected for validation by semi-quantitative real-time PCR. The targets of miRNAs were predicted by TargetScan program. RESULTS: 856 human miRNAs were analyzed by miRNA microarray. 25 differentially expressed miRNAs were found in L02T cells compared with L02 cells, with 15 upregulated and 10 downregulated. Among these miRNAs, miR-320a, miR-638 and miR-98 were validated by quantitative real-time PCR. Moreover, predicted by TargetScan program, we identified 4 genes that were potentially targeted by miR-638.These genes might be associated with hepatic cell transformation induced by AFB1. CONCLUSION: 25 differentially expressed miRNAs were identified in AFBl-transformed L02T cells. These miRNAs may play important roles in the process of malignant transformation of human cells.关键词
黄曲霉毒素B1/永生化人肝细胞/细胞恶性转化/miRNAKey words
aflatoxin B1/ immortalized human hepatic cells/ malignant cell transformation/ miRNA分类
医药卫生引用本文复制引用
李志芳,李道传,王庆,刘彩霞,李文学,陈雯..黄曲霉毒素B1诱导的恶性转化肝细胞miRNA表达谱的变化[J].癌变·畸变·突变,2011,23(1):26-30,5.基金项目
国家自然科学基金项目(30800930),国家杰出青年基金(309250929) （30800930）
