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多环芳烃降解菌的筛选与降解能力测定

袁爽 梁成华 李凤梅 郭书海

生态学杂志2011,Vol.30Issue(2):315-319,5.
生态学杂志2011,Vol.30Issue(2):315-319,5.

多环芳烃降解菌的筛选与降解能力测定

Screening of PAHs-degrading bacterial strains and determination of their capability in degrading PAHs

袁爽 1梁成华 1李凤梅 2郭书海3

作者信息

  • 1. 沈阳农业大学,沈阳110866
  • 2. 中国科学院沈阳应用生态研究所,沈阳110016
  • 3. 中国科学院研究生院,北京100049
  • 折叠

摘要

Abstract

Eight PAHs-degrading bacterial strains were screened out from the enriched culture with PAHs-contaminated soils in Benxi City of Liaoning Province, Northeast China, and the capability of the strains in degrading phenanthrene, phyrene, and benzo[ a ] pyrene with an initial concentration of 50, 50, and 5 mg · L-1 in medium was determined after 28-day incubation at 28 ℃. Among the strains screened out, B3 showed the best degrading effect, with the degradation rate of phenanthrene, phyrene, and benzo [ a ] pyrene being 88.4%, 54. 0%, and 68.4%,while the degradation rate of these compounds by the mixed 8 strains was 87.7%, 35.3%, and 42.0%, respectively. The strain B3 was identified as Pseudomonas sp. , based on its physiological and biochemical characteristics and the sequence analysis of 16S rRNA.

关键词

多环芳烃/多环芳烃降解菌/微生物降解

分类

资源环境

引用本文复制引用

袁爽,梁成华,李凤梅,郭书海..多环芳烃降解菌的筛选与降解能力测定[J].生态学杂志,2011,30(2):315-319,5.

基金项目

国家自然科学基金项目(21047006)资助. (21047006)

生态学杂志

OA北大核心CSCDCSTPCD

1000-4890

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