癌变·畸变·突变2011，Vol.23Issue(1)：22-25,30,5.DOI:10.3969/j.issn.1004-616x.2011.01.006
三氧化二砷对肺癌移植瘤的放疗增敏效应
Radiosensitization effects of arsenic trioxide in lung cancer xenografts
摘要
Abstract
OBJECTIVE: To explore the effects of arsenic trioxide on radiosensitivity and the possible mechanism in lung cancer xenografts. METHODS: The tumor-bearing C57BL/6 mouse model was established by injecting Lewis lung cancer cells into right infra-axillary dermis. 40 mice were randomized into 4 groups: control group(peritoneal injection 0.9％ NS,0.2 ml); As2O3 group(peritoneal injection,2.0 mg/kg,0.2 ml As2O3); irradiation group(peritoneal injection 0.9％NS,0.2 ml + 6MeV Electron Beam, 10 Gy); As2O3 + irradiation group(peritoneal injection, 2.0 mg/kg, 0.2 ml As2O3 + 6MeVElectron Beam, 10 Gy). After experiments, mice were sacrificed, then the tumors were separated and weighed. Response to irradiation was evaluated by tumor-inhibiting rate. The expressions of VEGF and Ku70 mRNA were evaluated by RT-PCR.The protein levels of VEGF and Ku70 were measured by immunohistochemical staining. RESULTS: The Lewis lung cancerbearing model was successfully established in mice. As2O3 + irradiation could significantly increase the tumor-inhibiting rate and downregulate the expression of VEGF and Ku70 of irradiated carcinoma xenografts, (Compared with As2O3 group or irradiation group, P ＜ 0. 05). CONCLUSION: Arsenic trioxide could sensitize lung cancer xenografts to ionizing irradiation.This effect was probably mediated by downregulating the expressions of VEGF and Ku70.关键词
三氧化二砷/肺癌/放射敏感性Key words
arsenic trioxide/ lung cancer/ radiosensitivity分类
医药卫生引用本文复制引用
任庆兰,吴永忠,陈晓品,李少林,金丹..三氧化二砷对肺癌移植瘤的放疗增敏效应[J].癌变·畸变·突变,2011,23(1):22-25,30,5.基金项目
重庆市卫生局中医药科研计划项目(2008-2-31) （2008-2-31）
