癌变·畸变·突变2011，Vol.23Issue(1)：61-64,4.DOI:10.3969/j.issn.1004-616x.2011.01.016
普洱茶水提取物对丝裂霉素C诱发人成淋巴细胞株遗传损伤的影响
The effects of Pu'er tea on genetic damage induced by mitomycin C in human lymphoblast cell line
摘要
Abstract
OBJECTIVE: The study focused on the effects of the water extract of Pu'er tea on genetic damage induced by mitomycin C (MMC) in human lymphocyte cell line. METHODS: The lymphoblast cell line GM12593 was cultured in RPMI-1640 medium plus MMC (0.1 μ g/ml and different combinations of red and raw Pu'er tea (0～1 mg/ml)for 24 h. Cytochalasin B was added and cells were cultured for another 28 h, centrifuged and put on slides. The frequencies of micronucleated binucleated cells were analysed. RESULTS: Fermented (red) Pu'er tea (0.125 mg/ml, 0.25 mg/ml) and raw (green) Pu'er tea (0.125 mg/ml) significantly reduced the frequency of micronucleated binucleated cells induced by MMC (P＜0.05). The effect of fermented Pu'er tea in preventing genetic materials induced by MMC was slightly stronger.CONCLUSION: Pu'er tea was able to reduce the genetic damage induced by MMC.关键词
普洱茶/丝裂霉素C/微核/遗传损伤/人成淋巴细胞Key words
Pu'er tea/ mitomycin C/ micronucleus/ genetic damage/ human lymphoblast分类
医药卫生引用本文复制引用
梁碧洋,周滔,倪娟..普洱茶水提取物对丝裂霉素C诱发人成淋巴细胞株遗传损伤的影响[J].癌变·畸变·突变,2011,23(1):61-64,4.基金项目
云南省科技厅项目(2010ZC065) （2010ZC065）
