农药学学报2011，Vol.13Issue(1)：21-27,7.DOI:10.3969/j.issn.1008-7303.2011.01.04
辣椒疫霉对双炔酰菌胺的敏感性测定及其抗药突变体生物学性状研究
Study on the sensitivity of Phytophthora capsici to mandipropamid and biological characteristics of resistant mutants
摘要
Abstract
The sensitivity and resistance risk of Phytophthora capsici to mandipropamid were studied.The sensitivity of 173 P.capsici strains to mandipropamid was determined by mycelia growth inhibition method.The results demonstrated that EC50 values ranged from 0.002 7 μg/mL to 0.049 9 μg/mL,with a mean value of 0.021 9 ± 0.010 2 μg/mL.The sensitivity frequency of P.capsici to mandipropamid distributed as a single peak curve.The 10 strains were separately treated by UV-irradiating with colony point change and taming, with 7 and 1 resistant mutants to mandipropamid separately obtained in vitro by UV-irradiating and taming, the resistant levels ranged from 41.50 to 394 437.77 times.Mutation frequencies were 1.46％ and 1.43％, respectively.Some biological characteristics of mutants were studied in laboratory.The mutants exhibited diversity in mycelial growth rate, pathogenicity, sporulation ability, fitness and competition ability with their parental strains.The sporulation,fitness and competition of DX 2-3 20m and DX 2-3 F7 were higher than the parental strains, contributing to the formation of resistance strains.It was suggested that P.capsici had a moderate resistance risk to mandipropamid.关键词
辣椒疫霉/双炔酰茵胺/敏感性/抗性突变体/生物学性状Key words
Phytophthora capsici/ mandipropamid/ sensitivity/ resistant-mutant/ biological characteristics分类
农业科技引用本文复制引用
赵卫松,韩秀英,齐永志,王文桥,马志强,张金林,张小风..辣椒疫霉对双炔酰菌胺的敏感性测定及其抗药突变体生物学性状研究[J].农药学学报,2011,13(1):21-27,7.基金项目
河北省科技支撑计划(08220302D) （08220302D）
公益性行业(农业)科技专项(200903033). （农业）
