中国药房2011，Vol.22Issue(7)：623-625,3.
RP-HPLC法测定赤芍总苷原料中芍药苷和赤芍总苷的含量
Content Determination of Peoniflorin and Total Paeony Glycoside in Paeonia Materials by RP-HPLC
摘要
Abstract
OBJECTIVE: To establish determination methods of peoniflorin and total paeony glycoside in paeonia materials.METHODS: The content of peoniflorin and total paeony glycoside were determined by RP-HPLC. The determination was performed on YMC-Pack ODS-A (250 mm ×4.6 mm, 5 μm) with mobile phase consisted of acetonitrile-0.02 mol· L-1 NaH2PO4 (20:80) at detection wavelength of 230 nm and 227 nm. RESULTS: The linear range of peoniflorin and total paeony glycoside were 0.832～4.160 μg (r=0.999 3) and 0.022 4～0.112 0 μg (r=0.999 1), respectively. The average recoveries were 104.2％ and 105.2％ respectively. RSD were 1.96％ and 1.94％ (n=9). CONCLUSION: The method is simple and easy for determination and analysis of paeonia materials. The method is characterized with the features of accuracy,reproducibility and stability.关键词
赤芍总苷原料/芍药苷/反相高效液相色谱法/含量测定分类
医药卫生引用本文复制引用
朱金芳,马生军,包晓玮,金学胜..RP-HPLC法测定赤芍总苷原料中芍药苷和赤芍总苷的含量[J].中国药房,2011,22(7):623-625,3.基金项目
新疆农业大学教学研究项目(200927) （200927）
