中国药房2011，Vol.22Issue(7)：654-657,4.
仙茅随机扩增多态性DNA反应体系的优化
Optimization of the RAPD Reaction System for Curculigo orchioides
摘要
Abstract
OBJECTIVE: To study the optimization of RAPD reaction system for Curouligo orchioides. METHOD: Using genomic DNA extracted from C. orchioides as template, the effects of the main parameters of RAPD reaction system (Mg2+, dNTP,primer, template DNA, Taq DNA polymerase) on amplification results were tested by single factor experiment to determine its optimal levels. RESULTS: A suitable reaction system for C. orchioides was established, i.e. 25 μL amplification reactions system conraining l×PCR buffer, 2.0 mmol·L-1 Mg2+, 200 μmol· L-1 dNTP, 40 ng primer, 20 ng template DNA, 1 IU Taq DNA polymerase.CONCLUSION: This optimal reaction system can be used for RAPD-PCR amplification of C. orchioides. Obtained clear spectrum confirms the reliability of RAPD reaction system.关键词
仙茅/随机扩增多态性DNA技术/反应体系/优化分类
医药卫生引用本文复制引用
李隆云,陈大霞,李泉森..仙茅随机扩增多态性DNA反应体系的优化[J].中国药房,2011,22(7):654-657,4.基金项目
国家科技攻关计划课题(2001BA701A32 （）
2004BA604A04) （）
重庆市科技计划项目(6455) （6455）
