中国药房2011，Vol.22Issue(7)：630-632,3.
RP-HPLC法同时测定不同产地栀子中栀子苷、西红花苷-Ⅰ和西红花苷-Ⅱ的含量
Content Determination of Gardenoside,Crocin-Ⅰ and Crocin-Ⅱ in Gardenia jasminoides from Different Producing Areas by RP-HPLC
张留记 1刘钦松 2屠万倩 1刘怡2
作者信息
- 1. 河南中医学院,郑州市450008
- 2. 河南省中医药研究院,郑州市,450004
- 折叠
摘要
Abstract
OBJECTIVE: To establish a method for the content determination of gardenoside, crocin- Ⅰ and crocin- Ⅱ in Gardenia jasminoides, and to determine the content of 3 components in 14 batches of samples from different producing areas.METHODS: RP-HPLC method was used.A Thermo ODS C18(250 mn×4.6 mm,5 μm) column was used with the mobile phase consisted of acetonitrile-0.1％ phosphoric acid (gradient elution) at the flow rate of 1.0 mL·min-1.The UV detection wavelength was set at 238 nm for gardenoside and 440 nm for crocin- Ⅰ and crocin- Ⅱ , respectively.RESULTS: The linear ranges of gardenoside, crocin-Ⅰ and crocin-Ⅱ were 0.448～5.600 μg(r=0.999 2), 0.142～1.775 μg(r=0.999 8) and 0.026～0.325 μg(r=0.999 9), respectively.The average recovery rates of gardenoside, crocin- Ⅰ and crocin-Ⅱ were 99.69％ (RSD=2.44％, n=6), 98.26％ (RSD= 2.62％ ,n=6) and 102.94％ (RSD=3.13％ ,n=6), respectively.CONCLUSION: The method is sample, accurate and reproducible for the content determination of gardenoside, crocin- Ⅰ and crocin- Ⅱ simultaneously in G.jasminoides.关键词
栀子/栀子苷/西红花苷-Ⅰ/西红花苷-Ⅱ/反相高效液相色谱法/含量测定分类
医药卫生引用本文复制引用
张留记,刘钦松,屠万倩,刘怡..RP-HPLC法同时测定不同产地栀子中栀子苷、西红花苷-Ⅰ和西红花苷-Ⅱ的含量[J].中国药房,2011,22(7):630-632,3.
