中国药房2011，Vol.22Issue(7)：611-613,3.
响应曲面法优选金银花中绿原酸微波辅助提取工艺
Optimization of Microwave-assisted Extraction Processes of Chlorogenic Acid from Lonicerar japonica by Response Surface Methodology
张霞 1崔海燕 2贾晓艳 1王玉玲 1张志琪1
作者信息
- 1. 陕西师范大学化学与材料科学学院,西安市,710062
- 2. 宁夏医科大学药学院,银川市,750004
- 折叠
摘要
Abstract
OBJECTIVE: To optimize the microwave-assisted extraction process of chlorogenic acid from Lonicerare japonica and to analyze the anti-oxidation function of the extract.METHODS: The extraction technology of chlorogenic acid was optimized by response surface methodology using the concentration of methanol, ratio of material to liquid and extraction time as factors and taking the absorbance of extract at 327 nm and the scavenging rate of extract for free radical as response.The anti-oxidation function of the extract was analyzed by comparing the scavenging capacity of extract against DPPH· RESULTS: The optimal extraction process was as follows: 43％ menthol as solvent, the ratio of material to liquid was 40 ml·g-1, extracting for 8 min.The field of chlorogenic acid was 7.62％ The scavenging capacity of extract for DPPH· was direct correlation with the content of chlorogenic acid.CONCLUSION: The optimized extraction process is stable, reasonable and feasible with high field and short extraction time.Chlorogenic acid plays a major role in the anti-oxidation of extract of L.japonica.关键词
金银花/绿原酸/响应曲面法/微波辅助提取分类
医药卫生引用本文复制引用
张霞,崔海燕,贾晓艳,王玉玲,张志琪..响应曲面法优选金银花中绿原酸微波辅助提取工艺[J].中国药房,2011,22(7):611-613,3.
