发光学报2011，Vol.32Issue(1)：94-99,6.DOI:10.3788/fgxb20113201.0094
基于延迟荧光光谱F685/F730温度响应曲线的植物耐热性评价
The Application of The F685/F730 Ratio of Delayed Fluorescence Spectrum Temperature Curve in Heat Tolerance Evaluation
摘要
Abstract
In order to obtain the relationship between temperature-stress and delayed fluorescence (DF) emission spectra, in this paper, three different heat-resistant maizes were used as samples to experimentally study the plant DF emission DF spectra at different elevated temperatures ( 10 ～ 50 ℃ ). The experimental spectra were obtained by 660 nm excitation. Results showed that there are two distinct emission peaks in DF spectra,that is, the main peak at around 685 nm and the second peak at around 730 nm. The maximal values of DF emission spectra at 685 nm and 730 nm were marked as F685 and F730 , respectively. The experimental results illustrated that the F685/F730 ratio varied with the increase of temperature. It was also found that the F685/F730 ratio at 10 ℃ and 50 ℃, which was marked as V10 and V50, respectively, are obviously different. The V10/V50 ratio depended on the heat-resisting property of the samples. These results indicated that the higher the heatresisting property of the sample is, the lower the V10/V50 ratio is. Additionally, the longer temperature stress time is, the lower V10/V50 ratio is. Hence, we concluded that DF emission spectra vary with the temperaturestress, and the response curve of F685/F730 ratios of different samples display a similar law. Moreover, the responses of F685/F730 ratio of DF emission spectra to the temperature for the different heat-resistant Maize samples could be a new and useful judgment to identify and evaluate the heat-resisting property of maize.关键词
延迟荧光/延迟荧光光谱/温度胁迫/F685/F730/耐热性Key words
delayed fluorescence/ delayed fluorescence spectra/ temperature stress/ F685/F730/ heat tolerance property分类
农业科技引用本文复制引用
李瑛,许文海,董丽丽,邢达..基于延迟荧光光谱F685/F730温度响应曲线的植物耐热性评价[J].发光学报,2011,32(1):94-99,6.基金项目
国家自然科学基金(60774056) （60774056）
中央高校基本科研业务费专项基金(2009QN032)资助项目 （2009QN032）
