工业微生物2011，Vol.41Issue(1)：36-40,5.DOI:10.3969/j.issn.1001-6678.2011.01.008
基因组重排与传统育种相结合选育大观霉素高产菌株
Genome shuffling combined traditional mutagenesis for improving Streptomyces spectabilis
摘要
Abstract
The genome shuffling combined the traditional mutagenesis was applied to improve Streptomyces spectabilis for producing spectinomydn. Firstly the UV-irradiation was adopted to mutate the protoplasm of Streptomyces spectabilis,and then the mutant strains with high specinomycin production were carried to protoplast fusion by twice. The fusant strain BR2-1 was screened out and sent to NTG-induced mutation. The neomycin-resistant strains X40-2, X40-5 and streptomycin-resistant stains L30-1, L50-1 were selected to send to the second genome shuffling from the NTG-treated filial strains. Lastly a fusant strain LXR1H-8 producing spectinomycin as high as 4 405 U/mL was obtained, resistant to both neomycin and streptomycin. The yield of spectinomycin was increased by 486.3％ comparing with that of the original strain XZ-4.关键词
大观霉素/基因组重排技术/传统诱变技术Key words
spectinomycin/ genome shuffling/ traditional mutagenesis引用本文复制引用
夏云重,裘娟萍,张正波,辛波波,方声..基因组重排与传统育种相结合选育大观霉素高产菌株[J].工业微生物,2011,41(1):36-40,5.基金项目
浙江省重中之重学科开放研究基金. （）
