山东农业大学学报（自然科学版）2011，Vol.42Issue(1)：6-10,5.
假俭草体细胞抗寒突变体的筛选及其鉴定
SCREEN AND IDENTIFICATION OF THE COLD- RESISTANCE SOMATIC MUTANT OF CENTIPEDEGRASS
摘要
Abstract
Poor cold - resistance of centipedegrass is one of the major factors limiting its wide application. The objective of this study is to obtain cold - resistant somatic mutants from the seed of ‘ Cuilù No. 1 ' centipedegrass by induction and screening at low temperature. The callus induced by seeds of centipedegrass was cultivated at the low temperature of 0℃ for twenty - four days during the process of subculture and three pieces of survival callus tissues were obtained. somatic mutant is obtained after survival callus subculture proliferation, differentiation, rhizogenesis as well as transplantation. Somatic mutant and the control. LT50of were -6.946℃ and - 5.851℃, respectively, which was significant difference. The results of SRAP markers demonstrated that there is characteristic bands at 200 bp of somatic mutant and indicated that the somatic mutants of centipedegrass were genetically stable comparing with that of the control plant on molecular level. This experimentation may provide effective means for screening of cold - resistance somatic mutant of plants.关键词
假俭草/体细胞变异/半致死温度Key words
Centipedegrass/ somatic mutant/ LT50/ SRAP分类
农业科技引用本文复制引用
袁学军,廖丽,李艳丽,王志勇,陈永敢..假俭草体细胞抗寒突变体的筛选及其鉴定[J].山东农业大学学报（自然科学版）,2011,42(1):6-10,5.基金项目
国家自然科学基金项目(31060266)、海南省自然科学基金项目(310031)、江西省教育厅青年科学基金项目(GJJ09627)和景德镇市科技局2008年度社会发展基金项目. （31060266）
