山东农业大学学报（自然科学版）2011，Vol.42Issue(1)：35-40,6.
大蒜休眠芽愈伤诱导和植株再生
DEVELOPMENT OF A PLANT REGENERATION SYSTEM FROM DORMANT BUD - DERIVED CALLUSES OF GARLIC (ALLIUM SATIVUM L.)
陈光宙 1袁学军 1李艳丽 1陈永敢1
作者信息
- 1. 琼州学院生命科学与技术学院,三亚,572000
- 折叠
摘要
Abstract
Plant regeneration systems have been successfully established with dormant bud of Jin garlic No. 3'as explant. The basal culture medium was MS, The optimum medium of callus induction was MS medium with 2,4 - D2.0 mg/L, the rate of callus induction was 99.5％. The rate of yellow granule callus induction could be 100％, the optimum medium of callus subculture was MS medium with2,4 - D 2.0 mg/L. The rate of yellow granule callus differentiation could be 98.6％ on optimum medium with 6 - BA 3.0 mg/L. The optimum medium of shoot growth was MS medium plus 6 - BA1.0 mg/L, KT1.0 mg/L, GA0. 2 mg/L and 0. 1NAA mg/L.The optimum temperature of shoot growth was20℃. The rooting rate was 92.4％ on optimum rooting medium with NAA0.2 mg/L. The survival rate of plant transplanting was 92.4％ after transferred to pots with garden soil. This experimentation may provide basic reference for screening of somaclonal variants and genetic transformation of garlic.关键词
金蒜3号/休眠芽/愈伤组织/植株再生Key words
Jin garlic No.3/ dormant bud/ callus/ plant regeneration分类
农业科技引用本文复制引用
陈光宙,袁学军,李艳丽,陈永敢..大蒜休眠芽愈伤诱导和植株再生[J].山东农业大学学报（自然科学版）,2011,42(1):35-40,6.
