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Clostridium autoethanogenum的生长培养基优化

郭颖 许敬亮 徐惠娟 袁振宏

可再生能源2011,Vol.29Issue(1):53-56,4.
可再生能源2011,Vol.29Issue(1):53-56,4.

Clostridium autoethanogenum的生长培养基优化

Medium optimization for the growth of Clostridium autoethanogenum

郭颖 1许敬亮 1徐惠娟 1袁振宏1

作者信息

  • 1. 中国科学院,广州能源研究所,可再生能源与天然气水合物重点实验室,广东,广州,510640
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摘要

Abstract

Statistical strategy was applied to optimize the medium for the growth of Clostridium autoethanogenum, which can convert H2, CO and CO2 into ethanol and acids. The main medium components were firstly divided into eleven groups according to their different effects. According to Plackett-Burman design, Yeast extract was screened to be the only significant factor affecting the growth of C. autoethanogenum and the relative advisable concentrations of other medium compositions were suggested. The optimal concentration of 1.50 g/L for yeast extract was established. Under those conditions, 399.46 mg/L of Clostridium autoethanogenum per liter were finally obtained. This optimized medium for the growth of Clostridium autoethanogenum could be applied in two-step CSTRS for ethanol production from syngas.

关键词

Clostridium autoethanogenum/Plackett-Burman设计/合成气发酵/培养基优化

Key words

Clostridium autoethanogenum/ Plackett-Burman design/ syngas fermentation/medium optimization

分类

能源科技

引用本文复制引用

郭颖,许敬亮,徐惠娟,袁振宏..Clostridium autoethanogenum的生长培养基优化[J].可再生能源,2011,29(1):53-56,4.

基金项目

中国科学院知识创新工程项目(KGCX2-YW-335) (KGCX2-YW-335)

国家高技术研究发展计划("863"计划)(2007AA05Z406 ("863"计划)

2007AA100702-4). ()

可再生能源

OA北大核心CSTPCD

1671-5292

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