水产学报2011，Vol.35Issue(1)：104-115,12.DOI:10.3724/SP.J.1231.2011.17121
生物强化反应器净化循环养殖废水的研究
Purifying effects of enhanced bioreactor on the wastewater in recirculating aquaculture systems
摘要
Abstract
An enhanced bioreactor was designed according to the highly effective interception principle of biofilm in recirculating aquaculture systems (RAS)by inoculating nitrifying and denitrifying bacteria to the elastic fiber carriers on which biofilms could be observed in situ 6 weeks after inoculating. The impact of hydraulic retention time (HRT) and water temperature (WT) on the purifying effects of enhanced bioreactor on wastewater of RAS was investigated. The result showed that HRT and WT significantly impacted the purifying effect of the bioreactor on wastewater( P ＜ 0.05). After analysis of significant differences between HRT and WT within removal rate of CODMn, TN and NH+4 -N, and considering run-time, cost and operational easiness of bioreactor,it was concluded that 18 h of HRT and 30 ℃ were the best operating conditions. The bioreactor was operated stably for 25 days under these conditions, the average removal rate of CODMn, TN and NH+4-N reached 44.18％ ,51.31％ and 82.08％, respectively. In addition, the cultivable microorganisms in the biofilm of the enhanced bioreactor were detected regularly throughout the 25 days of experimental time. As the biofilm developed, the biomass of all kinds of cultivable microorganisms rose, average levels of nitrifying bacteria grew from 35.91 × 104 CFU/mg to 89.43 × 104 CFU/mg, nitroso-bacteria from 25.75 × 104 CFU/mg to 61.65 × 104 CFU/mg and denitrifying bacteria from 14.23 × 104 CFU/mg to 100.95 × 104 CFU/mg. Nitrifying and nitroso-bacteria dominated among cultivable microorganisms(59.95％ -81.25％ ).Denitrifying bacteria were constantly adsorbed, colonized and successfully reproduced on the surface of elastic fiber carriers, which made biofilms more mature and stable (the removal rate of TN and NH+4-N reached respectively 51. 31％ and 82. 08％ ). The results of present study revealed that the enhanced bioreactor plays an important role in the removal of CODMn, TN and NH+4 -N in wastewater of RAS.关键词
生物反应器/微生物载体/水力停留时间/温度Key words
bioreactor/ microorganism carrier/ hydraulic retention time( HRT)/ water temperature Corresponding author: LU Kai-hong. E-mail: lukaihong@ nbu. edu. cn分类
资源环境引用本文复制引用
钱伟,陆开宏,郑忠明,吕镇梅,谢丽凤,张克鑫..生物强化反应器净化循环养殖废水的研究[J].水产学报,2011,35(1):104-115,12.基金项目
国家"八六三"高技术研究发展计划(2007AA10Z409) （2007AA10Z409）
宁波大学2010年优秀学位论文培育基金(PY20100012) （PY20100012）
