医药导报2011，Vol.30Issue(2)：190-193,4.DOI:10.3870/yydb.2011.02.016
拳参总黄酮的纯化研究
Studies on Purificating Process of the Total Flavonoids of Polygonum Bistorta L.
摘要
Abstract
Objective To provide a reference to further development and ulitization of the total flavanoids of Polygonum bistorta L. . Methods By the static and dynamic adsorption experiments, six types of macroporous adsorption resins( D101, HPD100,HPD400,HPD500, HPD600, AB-8) were evaluated and compared for the absorption and disaborption to find the optimum condition for the total flavanoids purification, and Rutin was used for the reference substance, 10％ A1C13 for the color reagent to determine the content of the total flavanoids of Polygonum bistorta L by UV-spectrophotometric method. Results The HPD400 macroporous adsorption resin possessed optimum adsorption and elution parameters. The best technological conditions were adding 9.28 mg · mL-1 of sample with pH 3.0, being eluted with 2.9 multiple column volume of distilled water, 4.3 multiple column volume of 55％ ethanol. The purified flavoniods was about 45％ of total unpurified flavoniods. Conclusion The HPD400 macroporous adsorption resin fits for purifying flavoniods from Polygonum bistorta L. . This method was simple, practicable and effective.关键词
拳参/总黄酮/纯化分类
医药卫生引用本文复制引用
王桂玲,房建强,赵雪梅,王松..拳参总黄酮的纯化研究[J].医药导报,2011,30(2):190-193,4.
