烟台大学学报（自然科学与工程版）2011，Vol.24Issue(1)：48-53,6.
鹿角菜18S rDNA序列分析及其系统发生分析
Nucleotide Analysis and Molecular Phylogenetic Affinity of 18S rDNA of Silvetia siliquosa
摘要
Abstract
DNA of Silvetia siliquosa is extracted and the 18S ribosome DNA sequence is amplified using poly chain reaction. The sequence result shows that it is composed of 1733 nucleotides in which the A, T, C and G contents are 25. 45％ , 26. 72％ , 26.72％ and 21.12％ , respectively. The nucleotides has been submitted to the database of Gene Bank, and accession number is GQ433994. Aligning with other sequences of 18S rDNA in Phaeophyceae , it reveals that there are 184 variable sites, 161 parsimonious-information sites and 23 singleton sites. Moreover, the results show that the number of base transition, transversion and transition-transversion ratio are 44, 30 and about 1.5, respectively. The phylogenetic tree, which inferred from the 18s rDNA sequence alignment by neighbor-joining method , shows that the 18S ribosomal gene is so conserved that it illustrats a good prospect of application in algae identification and classification. PLACE database prediction result shows that some cis-acting regulatory DNA elements such as dehydration-responsive elements, light-regulated transcription elements, Ca2+-responsive elements, et al. , are found in the nucleotide sequence. Therefore, it indicates that 18S ribosomal RNA gene may probably take part in some important regulation pathways in cell.关键词
鹿角菜/18S rDNA/转录因子/系统发生分类
生物科学引用本文复制引用
刘玮,李美真,詹冬梅,丁刚,吴海一..鹿角菜18S rDNA序列分析及其系统发生分析[J].烟台大学学报（自然科学与工程版）,2011,24(1):48-53,6.基金项目
国家863高技术研究发展计划(2006AA10A416) （2006AA10A416）
山东省科技攻关计划(2006GG2205011) （2006GG2205011）
山东省良种工程重大项目(2005～2009). （2005～2009）
