医药导报2011，Vol.30Issue(2)：148-152,5.DOI:10.3870/yydb.2011.02.004
菝葜药理活性部位的纯化
Purification of Pharmacological Active Parts in Smilax china L.
摘要
Abstract
Objective To find out the best process for purification of pharmacological active parts from Smilax china L. with macroporous adsorptive resins and biochemical integrity. Methods D101 macroporous adsorptive resin was used to purify compounds and the corresponding chromatogram maps were set up with DAD-HPLC according to the content of total steroidal saponins. Results The best procedure was that eluted the compounds with distilled water and 3BV 30％ ethanol to eliminate the impurities, collected the 4BV 70％ ethanol eluent to gain the steroid saponins and wash the resin with 95％ ethanol at last. Conclusion The purified substances obtained from this process are with high purity and good biochemical integrity.关键词
菝葜/活性部位/甾体总皂苷/大孔吸附树脂/三级管阵列检测器-高效液相色谱法/纯化物分类
医药卫生引用本文复制引用
胡丽玲,廖婧,于丽秀,华小黎,陈东生..菝葜药理活性部位的纯化[J].医药导报,2011,30(2):148-152,5.基金项目
湖北省科委重点攻关计划项目资助(基金编号:2002P1105) （基金编号:2002P1105）
