中国人兽共患病学报2011，Vol.27Issue(2)：112-116,123,6.
鳗源嗜水气单胞菌主要粘附素基因克隆表达及产物粘附功能分析
Cloning,expression and adhesive characterization of the major adhesion gene in Aeromonas hydrophila isolated from Anguilla anguilla
摘要
Abstract
Aeromonas hydrophila strain ZN1 was isolated from European eel (Anguilla anguilla).Its major adhesin gene (Mah) was amplified by PCR and the DNA sequence analysis showed that Mah had a full-lenghth open reading frame (ORF) of 1074 nt, encoding a 357-aa peptide, and which N-terminal 22 amino-acid was highly hydrophobic signal polypeptide.The homologous comparison proved that Mah had about 70％ homology with the other Mah sequences from A.hydrophila.The amplified Mah was inserted into vector pET-32a and transformed into E.coliDE3(BL21) for expression.The Mah-TrxA fusion protein was efficiently expressed in E.coliDE3 (BL21) which were recognized by mouse anti-ZN1-MOMP serum by ELISA and also Western-blotting to get the expected bands comparing with ZN1-MOMP, which was containing major adhesin.Adhesive assay indicated that ZN1-MOMP and Mah-TrxA fusion protein were able to inhibit the adhesive fuction of the bacteria and the rabbit anti-Mah-TrxA serum could significantly block the ZN1 to adhere to EPC monolayers.So that, we could have the conclusion that the cloned gene represented the major adhesin gene of strain ZN1, and the gene products shared similar antigenicity with the natural major adhesion.关键词
嗜水气单胞菌/主要粘附素/粘附/克隆表达分类
农业科技引用本文复制引用
庄培德,杨金先,吴学敏,龚晖,林天龙..鳗源嗜水气单胞菌主要粘附素基因克隆表达及产物粘附功能分析[J].中国人兽共患病学报,2011,27(2):112-116,123,6.基金项目
福建省重点计划项目2007N0033资助 （）
