首页|期刊导航|中国神经再生研究（英文版）|Oxidative modification of the molecular chaperone family in a PC12 cell model of Parkinson's disease induced by Z-lle-Glu(OtBu)-Ala-Leucinal
中国神经再生研究（英文版）2011，Vol.6Issue(2)：85-90,6.DOI:10.3969/j.issn.1673-5374.2011.02.001
Oxidative modification of the molecular chaperone family in a PC12 cell model of Parkinson's disease induced by Z-lle-Glu(OtBu)-Ala-Leucinal
Oxidative modification of the molecular chaperone family in a PC12 cell model of Parkinson's disease induced by Z-lle-Glu(OtBu)-Ala-Leucinal
摘要
Abstract
Previous studies have demonstrated that ubiquitin-proteasome system function is significantly decreased in the substantia nigra of Parkinson's disease patients.In the present study, proteasome inhibitor Z-Ile-Glu(OtBu)-Ala-Leucinal (PSI) was used to inhibit the function of the ubiquitin-proteasome system in PC12 cells to simulate Parkinson's disease.Oxidatively modified proteins were identified to determine pathogenesis of Parkinson's disease.Results demonstrated that 24 hours of 10 μmol/L PSI-treatment in PC12 cells simulated pathological characteristics of Parkinson's disease: neuronal degeneration and eosinophilic inclusion formation in neurons.In PSI-treated PC12 cells, three oxidative proteins and a molecular chaperone family member were detected: chaperonin containing t-complex polypeptide 1 subunit 3, glucose-regulated protein 58,and heat shock protein 70.This is the first study to demonstrate oxidative modification of a molecule family in a cell model of Parkinson's disease induced with PSI.关键词
molecular chaperones/pathogenesis/proteomics/protein arbonylation/Parkinson's disease/ubiquitin-proteasome systemKey words
molecular chaperones/pathogenesis/proteomics/protein arbonylation/Parkinson's disease/ubiquitin-proteasome system引用本文复制引用
Ying Zhang,Yimin Yang,Jing Bai,Ming Chang,Linsen Hu..Oxidative modification of the molecular chaperone family in a PC12 cell model of Parkinson's disease induced by Z-lle-Glu(OtBu)-Ala-Leucinal[J].中国神经再生研究（英文版）,2011,6(2):85-90,6.基金项目
the Scientific Research Foundation of Department of Science and Technology of Jilin Province, No. 200505200 （）
