中国人兽共患病学报2011，Vol.27Issue(2)：117-119,3.
结核分枝杆菌培养滤液蛋白21(CFP21)的基因克隆、表达及纯化
Cloning, expression and purification of the gene of antigen culture filtrate protein 21 of Mycobacterium tuberculosis
摘要
Abstract
In order to construct culture filtrate protein 21(CFP21) vector of Mycobacterium tuberculosis (Mtb) and express it in E.coli BL21, the cfp21 gene was amplified from Mtb H37 Rv genomic DNA using PCR in vitro, and inserted into vector PET-28a (+) to construct the recombinant plasmid.The recombinant plasmid was then under the procedure of transforming into E.coli DH5α, plasmid extracting, PCR amplificating, sequencing, and transforming into E.coli BL21.The recombinant plasmid was induced with IPTG and identified using SDS-PAGE.Then the protein was purified using His Bind purification kit.Results demonstrated that CFP21 has been constructed, expressed and purified successfully.The molecular weight was about 24KD and the form of expression was inclusion bodies.In conclusion, the target gene has been cloned into host bacterium and expressed successfully.The purified recombinant protein CFP21 paves the way for TB diagnosis and vaccine development in the future.关键词
结核分枝杆菌/CFP21/基因克隆/蛋白表达/纯化分类
医药卫生引用本文复制引用
谢富佳,谭继英,梁正羽,乔梅,祝秉东,吴玉敏,杨燕,章国平..结核分枝杆菌培养滤液蛋白21(CFP21)的基因克隆、表达及纯化[J].中国人兽共患病学报,2011,27(2):117-119,3.基金项目
卫生部艾滋病和病毒性肝炎等重大传染病防治科技重大专项(No.2008zx100030135) （No.2008zx100030135）
教育部"国家大学生创新性实验计划"项目资助(No.081073017) （No.081073017）
