首页|期刊导航|中国神经再生研究（英文版）|Changes in synaptic and extrasynaptic N-methyl-D-aspartate receptor-mediated currents at early-stage epileptogenesis in adult mice
中国神经再生研究（英文版）2011，Vol.6Issue(2)：118-124,7.DOI:10.3969/j.issn.1673-5374.2011.02.007
Changes in synaptic and extrasynaptic N-methyl-D-aspartate receptor-mediated currents at early-stage epileptogenesis in adult mice
Changes in synaptic and extrasynaptic N-methyl-D-aspartate receptor-mediated currents at early-stage epileptogenesis in adult mice
摘要
Abstract
Previous reports have shown that N-methyl-D-aspartate (NMDA) receptors are extensively involved in epilepsy genesis and recurrence.Recent studies have shown that synaptic and extrasynaptic NMDA receptors play different, or even opposing, roles in various signaling pathways, including synaptic plasticity and neuronal death.The present study analyzed changes in synaptic and extrasynaptic NMDA receptor-mediated currents during epilepsy onset.Mouse models of lithium chloride pilocarpine-induced epilepsy were established, and hippocampal slices were prepared at 24 hours after the onset of status epilepticus.Synaptic and extrasynaptic NMDA receptor-mediated excitatory post-synaptic currents (NMDA-EPSCs) were recorded in CA1 pyramidal neurons by whole-cell patch clamp technique.Results demonstrated no significant difference in rise and delay time of synaptic NMDA-EPSCs compared with normal neurons.Peak amplitude, area-to-peak ratio,and rising time of extrasynaptic NMDA-EPSCs remained unchanged, but decay of extrasynaptic NMDA-EPSCs was faster than that of normal neurons.These results suggest that extrasynaptic NMDA receptors play a role in epileptogenesis.关键词
N-methyl-D-aspartate receptor/excitatory postsynaptic current/epilepsy/epileptogenesis/hippocampusKey words
N-methyl-D-aspartate receptor/excitatory postsynaptic current/epilepsy/epileptogenesis/hippocampus引用本文复制引用
Juegang Ju,Sheng-tian Li..Changes in synaptic and extrasynaptic N-methyl-D-aspartate receptor-mediated currents at early-stage epileptogenesis in adult mice[J].中国神经再生研究（英文版）,2011,6(2):118-124,7.基金项目
Shanghai Pujiang Program, No. 06PJ14053 （）
the National Natural Science Foundation of China, No. 30600177 （）
the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry （）
Doctoral Fund of Ministry of Education of China, No. 20070248083 （）
and Shanghai Leading Academic Discipline Project, No. B205 （）
