中国药理学与毒理学杂志2011，Vol.25Issue(1)：72-76,5.DOI:10.3867/j.issn.1000-3002.2011.01.014
七氟烷和异氟烷急性暴露对SD新生大鼠海马的神经毒性作用
Neurotoxic effects of sevoflurane and isoflurane acute exposure on hippocampus of neonatal SD rats
摘要
Abstract
OBJECTIVE To investigate the toxic effects and mechanisms of acute exposure to inhaled anesthetics of sevoflurane and isoflurane on hippocampus in neonatal SD rats. METHODS SD male rats of 7-day-old were exposed to 3.6 ％ sevoflurane and 2.3％ isoflurane respectively for 6 h. TUNEL and activated caspase 3 immunohistochemical staining were used to detect neuron apoptosis. Transmission electron microscope was use for morphological observation of hippocampus neurons. Western blotting was applied to check the expression of activated caspase 3. RESULTS Compared with normal control group 2 ± 2, neuron apoptosis was more remarkable in sevoflurane and isoflurane groups; the ammount of neuron apoptosis increased significantly to 13 ± 2 and 22 ± 5, respectively. There was statistical significance in neuron apoptosis number between sevoflurane and isoflurane groups. Misshapen nuclei, swelling mitochondria and endoplasmic reticula in sevoflurane and isoflurane groups were observed under an electron microscope. Western blotting showed that expression of activated cleaved caspase 3 increased significantly( P ＜ 0.05 )and isoflurane was inclined to cause more severe apoptosis neurodegeneration than sevoflurane (P ＜ 0.05 ). CONCLUSION Acute exposure to sevoflurane and isoflurane could induce apoptosis neurodegeneration. Isoflurane could cause more insults to brain of rats than sevoflurane at an equivalent concentration.关键词
七氟烷/异氟烷/海马/细胞凋亡/神经毒性分类
医药卫生引用本文复制引用
余梓浦,王佩芳,李纯,林函,康定鑫,狄美琴,连庆泉..七氟烷和异氟烷急性暴露对SD新生大鼠海马的神经毒性作用[J].中国药理学与毒理学杂志,2011,25(1):72-76,5.基金项目
浙江省自然科学基金(Y206467) （Y206467）
国家自然科学青年基金(30800323) （30800323）
温州市科技局项目(Y20080028) （Y20080028）
温州市科技局项目(Y20080032) （Y20080032）
