中国中西医结合杂志2011，Vol.31Issue(2)：218-222,5.
川楝素通过线粒体途径诱导人肝癌细胞凋亡
Human Hepatocarcinoma Cell Apoptosis Induced by Toosendanin through Mitochondria-dependent Pathway
摘要
Abstract
Objective To explore the effects of toosendanin in inducing apoptosis of human hepatocarcinoma cell line SMMC-7721 and Hep3B, and its influence on the related genes, Bcl-2, Bax and Fas.Methods The inhibitory rate of cell proliferation and cell growth curve were detected by MTT assay; morphological changes of cells were observed by inverted microscope; early stage apoptosis rate were detected by Annexin V-FITC/PI assay; relative activities of Caspase-3,-8 and-9 were analyzed by spectrophotometry; and the expressions of Bcl-2, Bax and Fas were detected using immunohistochemistry assay.Results Toosendanin presented significant inhibitory effect on proliferation of hepatocarcinoma cells in a time- and dose-dependent manner.After toosendanin treatment, the amount of cells was significantly reduced, shrunk in size and rounded in shape, with decreased adhesion ability.The apoptosis rates of SMMC-7721 cells and Hep3B cells treated with 0.5 μmol/L toosendanin for 72 h were 21.55％ and 18.35％ respectively, which were reduced after z-VAD-fmk ( inhibitor of Caspase) treatment.The activities of Caspase-3,-8 and -9 all markedly enhanced after treatment in SMMC-7721 cells, while in Hep3B cells, activities of Caspase-3 and -9 enhanced, but that of Caspase-8 unchanged.As compared with the control group, after toosendanin treatment, expression of Bcl-2 decreased, and that of Bax and Fas increased in SMMC-7721 cells; but in Hep3B cells the expression of Bcl-2 decreased, that of Bax increased, and expression of Fas unchanged.Conclusions Toosendanin could inhibit the proliferation and induce the apoptosis of both P53 + and P53- human hepatocarcinoma cells, which involved the participation of mitochondria-dependent pathway.So it may be a kind of natural anti-cancer drug, playing its effect through P53 independent pathway.关键词
川楝素/凋亡/SMMC-7721/Hep3B/P53引用本文复制引用
王鹏,何於娟,王进,姜慧,王虹,李忱炜,贺潇,崔瑾,董杰,刘小玲..川楝素通过线粒体途径诱导人肝癌细胞凋亡[J].中国中西医结合杂志,2011,31(2):218-222,5.基金项目
重庆市科委自然科学基金计划资助项目(No.2009BB5258) （No.2009BB5258）
重庆医科大学校级课题资助项目(No.XBYB2008086) （No.XBYB2008086）
