中国中西医结合杂志2011，Vol.31Issue(2)：228-232,5.
雷公藤红素对白血病细胞Akt信号通路的影响及在细胞凋亡中的作用
Effect of Celastrol on Akt Signaling Pathway and Apoptosis of Leukemic K562 Cell Line
摘要
Abstract
Objective To research the effect and route of celastrol on Akt signaling pathway of human leukemia cell line K562 apoptosis. Methods The activities of K562 proliferation cells were detected by MTT assay; cell apoptosis was detected by Hoechst 33258 staining assay, DNA fragmentation assay, and Annexin V/PI double-labeled cytometry; the expression and phosphorylation level of Caspase family members and AKT signaling pathway related proteins were determined by Western blot before and after celastrol treatment, and further the effect of AKT signaling pathway on celastrol-induced-apoptosis was analyzed. Results K562 cell proliferation was inhibited by celastrol in a time- and dose-dependent manner, with the IC50 value for24 h of (2. 15 ±0. 11 ) μmol/L. Celastrol induced K562 cells apoptosis in a dose-dependent manner, apparent DNA fragmentation and typical apoptotic morphological changes, and accompanied Caspase-3, 8 activation in the apoptosis process were shown after cells were treated with 2. 0 μmol/L celastrol for 24 h. And the celastrol induced apoptosis could be blocked by 50 μmol/L z-VAD-fmk (caspase inhibitor), but augmented by 25 μmol/L WORT (PI3K-Akt inhibitor). Moreover, Celastrol decreased the expressions of p-Akt, survivin and Bcl-2 in the Akt signaling pathway. Conclusions Celastrol inhibited the proliferation of K562 cells and induced cell apoptosis by way of activating caspase cascade. The decreased level of Akt phosphorylation during celastrol-induced-apoptosis process suggested that celastrol acted synergistically with PI3K-Akt inhibitors in K562 cell apoptosis inducing.关键词
雷公藤红素/K562细胞/Akt/Caspase-3/Caspase-8引用本文复制引用
王晓南,吴青,杨旭,张连生,吴一品,卢聪..雷公藤红素对白血病细胞Akt信号通路的影响及在细胞凋亡中的作用[J].中国中西医结合杂志,2011,31(2):228-232,5.基金项目
湖北省教育厅基金资助项目(No. B20101107) （No. B20101107）
