作物学报2011，Vol.37Issue(2)：362-368,7.DOI:10.3724/SP.J.1006.2011.00362
陆地棉两个同源基因GhBlind的克隆与表达分析
Isolation and Expression Analysis of Two GhBlind Homologs in Upland Cotton (Gossypium hirsutum L.)
摘要
Abstract
In higher plants, Blind homologs play an important role in plant architectures.Two Blind orthologs, GhBlind1 and GhBlind2, were isolated from cDNA of the shoot apex of upland cotton (Gossypium hirsutum L.) by homologous cloning strategy.GenBank accession number is HQ115643 for GhBlind1, and HQ115644 for GhBlind2.Similar to other Blind homologs, GhBlind1 and GhBlind2 were consisted of three exons and two introns, and encoded 359 and 262 amino acids, respectively.Based on the RT-PCR analysis, it was found that GhBlind1 expressed in root, leaf, and tender fiber, and predominantly in shoot apical meristem (SAM) and shoot apex, but not in stem; while GhBlind2 expressed in root, stem, leaf, SAM and shoot apex, but not in tender fiber.Using recombinant PCR technology, expression vectors pSuper-gus-b1 and pSuper-gus-b2 were constructed and used for Agrobacterium-mediated transient expression by transformation of embryogenic callus of upland cotton cv.Coker 201.Histochemical assays of GUS activity showed that after co-cultivation of embryogenic callus for four days, positive events were more obvious in pSuper-gus-b2 than in pSuper-gus-b1.The alignments between GhBlind1, GhBlind2 and the domain of Blind homologs showed the identities of 94％ and 91％, respectively.The results of phylogenetic tree showed that GhBlind1 and GhBlind2 were genetically divergent from most of the other R2R3-MYB members in Gossypium hirsutum L.We speculated that GhBlind1 and GhBlind2 are probably related with the development of lateral branch in upland cotton.关键词
棉花/Blind/同源克隆/表达分析引用本文复制引用
熊冠军,徐芹,华金平..陆地棉两个同源基因GhBlind的克隆与表达分析[J].作物学报,2011,37(2):362-368,7.基金项目
本研究由教育部新世纪优秀人才支持计划项目(NCET-06-0106),国家高技术研究发展计划(863计划)重大专项(2009AA101104),教育部长江学者和创新团队发展计划和高等学校科学创新引智计划(111-2-03)资助. （NCET-06-0106）
