中国农业科学2011，Vol.44Issue(1)：20-27,8.DOI:10.3864/j.issn.0578-1752.2011.01.003
中国棉花主栽品种DNA指纹图谱构建及SSR标记遗传多样性分析
Construction of DNA Fingerprinting and Analysis of Genetic Diversity with SSR Markers for Cotton Major Cultivars in China
摘要
Abstract
[ Objective] The aim of this study was to construct a DNA fingerprinting database of 32 cotton major cultivars from three main cotton regions (including eight main cotton production provinces), and the genetic diversity was analyzed based on simple sequence repeats (SSR). [Method] Thirty six evenly distributed SSR primer pairs with high polymorphisms and good repeatability were successfully screened out from 214 candidates to construct the fingerprinting database. [ Result] Among the 32 varieties, 36 primer pairs had 142 polymorphic genotypes, and 3.94 genotypes were detected by each SSR primer pair on an average with the range from 2 to 11. Ten cultivars had specific genotypes by 9 primer pairs. Thirty two major cultivars could be identified by 5 primer combinations at least. The genetic diversity analyzed by the software ofNTSYS-pc V 2.10 indicated that Yangtze River valley cotton region had the highest polymorphic level, followed by Xinjiang, and the lowest was the varieties from the Yellow River valley. The genetic basis of conventional cultivars was narrower than that of hybrids. [ Conclusion ] Core primer combination method is better than distinct band method for the construction of DNA fingerprinting of cotton major cultivars in China. The genetic relationship of the cotton cultivars derived from the same district is similar to a certain extent.关键词
棉花/主栽品种/SSR/DNA指纹图谱/遗传多样性引用本文复制引用
匡猛,杨伟华,许红霞,王延琴,周大云,冯新爱..中国棉花主栽品种DNA指纹图谱构建及SSR标记遗传多样性分析[J].中国农业科学,2011,44(1):20-27,8.基金项目
中央级公益性科研院所基本科研业务费专项重点项目(SJA0904)、农业部全国棉种质量抽查项目(农办农[2007]106号)、转基因生物新品种培育科技重大专项(2008ZX08013-001) （SJA0904）
